Effect of polypeptides in bee venom on growth inhibition and apoptosis induction of the human hepatoma cell line SMMC-7721 in-vitro and Balb/c nude mice in-vivo
Effect of polypeptides in bee venom on growth inhibition and apoptosis induction of the human hepatoma cell line SMMC-7721 in-vitro and Balb/c nude mice in-vivo
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DOI：
10.1211/jpp.58.1.0010
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发表时间：
2006-01-01
影响因子：
3.3
通讯作者：
Zhang, AG
中科院分区：
文献类型：
--
作者：
Hu, HY;Chen, DW;Zhang, AG
蜂毒多肽(PBV)对人肝癌细胞株SMMC-7721有明显的生长抑制作用。对细胞死亡机制的分析表明，PBV诱导了细胞的凋亡。SMMC-7721细胞暴露于PBV(10.0微克·毫升~(-1))后，细胞形态无明显变化。四甲基偶氮唑蓝(3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium)比色法分析表明，PBV的细胞毒作用具有时间和时间依赖性。Ki67免疫组织化学检测结果显示，PBV(10.0µg·mL~(-1))可抑制SMMC-7721细胞的增殖。膜联蛋白V、碘化丙啶染色和DNA片段化分析证实细胞死亡。在体内实验中，PBV(1.5或3 mg·kg~(-1))对裸鼠SMMC-7721细胞的生长有明显的抑制作用。这些发现表明，PBV可以作为一种抗肿瘤的化疗药物。
Polypeptides in bee venom (PBV) produced a significant growth inhibition against SMMC-7721 human hepatoma cell line. Analysis of the mechanisms of cell death indicated that PBV induced an apoptotic cell death. SMMC-7721 cells exposed to PBV (10.0 mu g mL(-1)) produced an insignificant morphological change. Analysis of the cytotoxicity with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) assay confirmed that the cytotoxic effects of PBV were close- and time-dependent. The result of Ki67 immunohistochemistry demonstrated that the proliferation of SMMC-7721 cells treated with PBV (10.0 mu g mL(-1)) was inhibited. The apoptotic cell death was then confirmed by annexin V, propidium iodide staining and DNA fragmentation analysis. in in-vivo experiments, treatment with PBV (1.5 or 3 mg kg(-1)) resulted in a significant retardation of SMMC-7721 cell growth in Balb/c nude mice. These findings suggested that PBV could be used as a chemotherapeutic agent against tumours.