EFFECT OF RU486 ON DEVELOPMENT AND IMPLANTATION OF RAT EMBRYOS
EFFECT OF RU486 ON DEVELOPMENT AND IMPLANTATION OF RAT EMBRYOS
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DOI：
10.1002/mrd.1080290405
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发表时间：
1991-08-01
影响因子：
2.5
通讯作者：
CROXATTO, HB
中科院分区：
文献类型：
--
作者：
ROBLERO, LS;CROXATTO, HB
本研究评价了性交后应用抗孕激素RU486对大鼠胚胎的运输、发育和着床的影响。分别于妊娠第1、1+2或4天皮下注射0.1、0.5、1.0、2.0或3.0 mg/只RU486。在怀孕第5天或第12天进行尸检。RU486可显著减少回收胚胎的数量，抑制胚胎的发育(第5天)，并减少植入胚胎的数量和大小(第12天)。第4天的治疗效果最差。RU486处理组大鼠的囊胚体外滋养层生长速度与对照组相当。从RU486处理的大鼠移植到同步未处理的假孕受体的囊胚在所有受体12天后都产生了植入的胚胎，尽管比率明显低于对照组。当剂量大于或等于1.0mg时，从对照孕鼠移植到RU486治疗假孕受体的囊胚无法完全植入。RU486在大鼠性交后处理的拦截机制包括生殖道胚胎丢失和植入前发育改变。RU486也会损害子宫内膜的容受性或子宫保留胚胎直到着床的能力。在这些影响下存活下来的胚胎可能会在母亲体内经历延迟着床。
This study evaluated the effects of postcoital treatment with the antiprogestin RU486 on transport, development and implantation of rat embryos. Doses of 0.1, 0.5, 1.0, 2.0, or 3.0 mg/rat of RU486 were injected subcutaneously on days 1, 1 + 2, or 4 of pregnancy. Autopsies were carried out on days 5 or 12 of pregnancy. RU486 provoked a significant dose-related reduction in the number of recovered embryos and inhibited their development (day 5) and decreased the number and size of implanted embryos (day 12). Treatment on day 4 was the least effective. Blastocysts recovered from RU486-treated rats exhibited comparable rate of trophoblastic outgrowth in vitro as the controls. Blastocysts transferred from RU486-treated rats to synchronous untreated pseudopregnant recipients yielded implanted embryos 12 days later in all recipients, although at a significantly lower rate than the controls. Blastocysts transferred from control pregnant rats to RU486-treated pseudopregnant recipients failed to implant completely when the dose was greater-than-or-equal-to 1.0 mg.The interceptive mechanism of postcoital treatment with RU486 in the rat involves loss of embryos from the reproductive tract and altered development prior to implantation. Endometrial receptivity or the ability of the uterus to retain the embryos until the time of implantation are also impaired by RU486. The embryos that survive these effects may experience delayed implantation in their mothers.