Lanthanide-Doped LiLuF4 Upconversion Nanoprobes for the Detection of Disease Biomarkers
Lanthanide-Doped LiLuF4 Upconversion Nanoprobes for the Detection of Disease Biomarkers
复制标题
用于检测疾病生物标志物的稀土掺杂 LiLuF4 上转换纳米探针
DOI：
10.1002/anie.201309503
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发表时间：
2014-01-27
影响因子：
16.6
通讯作者：
Chen, Xueyuan
中科院分区：
文献类型：
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作者：
Huang, Ping;Zheng, Wei;Chen, Xueyuan
镧系掺杂上转换纳米粒子（UCNPs）在生物应用中显示出巨大的潜力。探索新的基质材料以实现高效的上转换发光（UCL）输出是人们普遍关注的目标。在此，我们开发了一种独特的策略来合成新型的LiLuF₄:Ln³⁺核/壳UCNPs，其对于Er³⁺和Tm³⁺的绝对上转换量子产率分别高达5.0%和7.6%。基于我们定制的UCL生物检测系统，我们首次展示了LiLuF₄:Ln³⁺核/壳UCNPs作为灵敏的UCL生物探针用于检测一种重要的疾病标志物——人绒毛膜促性腺激素β亚基（β - hCG），其检测限为3.8 ng/mL，与正常人血清中的β - hCG水平相当。此外，我们将这些UCNPs用于癌细胞的概念验证计算机断层扫描成像和UCL成像，从而揭示了LiLuF₄:Ln³⁺ UCNPs作为疾病诊断中高效纳米生物探针的巨大潜力。
Lanthanide-doped upconversion nanoparticles (UCNPs) have shown great promise in bioapplications. Exploring new host materials to realize efficient upconversion luminescence (UCL) output is a goal of general concern. Herein, we develop a unique strategy for the synthesis of novel LiLuF4:Ln(3+) core/shell UCNPs with typically high absolute upconversion quantum yields of 5.0% and 7.6% for Er3+ and Tm3+, respectively. Based on our customized UCL biodetection system, we demonstrate for the first time the application of LiLuF4:Ln(3+) core/shell UCNPs as sensitive UCL bioprobes for the detection of an important disease marker beta subunit of human chorionic gonadotropin (beta-hCG) with a detection limit of 3.8 ng mL(-1), which is comparable to the beta-hCG level in the serum of normal humans. Furthermore, we use these UCNPs in proof-of-concept computed tomography imaging and UCL imaging of cancer cells, thus revealing the great potential of LiLuF4:Ln(3+) UCNPs as efficient nano-bioprobes in disease diagnosis.