Surfactant-associated protein a provides critical immunoprotection in neonatal mice
Surfactant-associated protein a provides critical immunoprotection in neonatal mice
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DOI：
10.1128/iai.01043-07
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发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Snyder, Jeanne M.
中科院分区：
文献类型：
--
作者：
George, Caroline L. S.;Goss, Kelli L.;Snyder, Jeanne M.
集合素、表面活性物质相关蛋白A(SP-A)和SP-D是先天免疫的组成部分，它们在出生前就已经存在。这两种蛋白质都能结合病原体，并有助于清除感染。作为新生儿免疫系统组成部分的SP-A和SP-D的意义尚未被研究。为了确定SP-A和SP-D在新生儿免疫中的作用，将野生型、SP-A缺失和SP-D缺失的小鼠饲养在细菌环境(玉米粉尘铺垫)或半湿热环境(纤维素纤维铺垫)中。与野生型和暴露在相同环境中的SP-D缺失型幼鼠相比，在玉米粉尘床上饲养的SP-A缺失型幼鼠具有显著的死亡率(P<；0.001)。SP-A缺失型仔鼠的死亡与明显的胃肠道病理有关，而与肺的病理关系不大。奄奄一息的SP-A基因缺失的新生小鼠出现芽孢杆菌。和肠球菌属。腹膜炎。当母亲或新生儿产生SP-A时，与母亲和幼崽完全缺乏SP-A的结果相比，新生儿存活率显著提高(P<；0.05)。可能保护新生儿的SP-A的重要来源包括新生儿的肺和胃肠道，但不包括母亲的哺乳期乳腺组织。此外，外源SP-A经口递送给SP-A缺失型母亲的新生SP-A缺失型幼鼠可以提高在玉米粉尘环境中的新生儿存活率。因此，缺乏SP-D并不影响新生儿的存活率，而由母亲或幼崽产生的SP-A或口服外源性SP-A显著降低了SP-A缺失型新生儿因环境感染而产生的新生儿死亡率。
The collectins surfactant-associated protein A (SP-A) and SP-D are components of innate immunity that are present before birth. Both proteins bind pathogens and assist in clearing infection. The significance of SP-A and SP-D as components of the neonatal immune system has not been investigated. To determine the role of SP-A and SP-D in neonatal immunity, wild-type, SP-A null, and SP-D null mice were bred in a bacterium-laden environment (corn dust bedding) or in a semisterile environment (cellulose fiber bedding). When reared in the corn dust bedding, SP-A null pups had significant mortality (P < 0.001) compared to both wild-type and SP-D null pups exposed to the same environment. The mortality of the SP-A null pups was associated with significant gastrointestinal tract pathology but little lung pathology. Moribund SP-A null newborn mice exhibited Bacillus sp. and Enterococcus sp. peritonitis. When the mother or newborn produced SP-A, newborn survival was significantly improved (P < 0.05) compared to the results when there was a complete absence of SP-A in both the mother and the pup. Significant sources of SP-A likely to protect a newborn include the neonatal lung and gastrointestinal tract but not the lactating mammary tissue of the mother. Furthermore, exogenous SP-A delivered by mouth to newborn SP-A null pups with SP-A null mothers improved newborn survival in the corn dust environment. Therefore, a lack of SP-D did not affect newborn survival, while SP-A produced by either the mother or the pup or oral exogenous SP-A significantly reduced newborn mortality associated with environmentally induced infection in SP-A null newborns.