Metal ions from S-PRG filler have the potential to prevent periodontal disease.
Metal ions from S-PRG filler have the potential to prevent periodontal disease.
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DOI：
10.1002/cre2.70
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发表时间：
2017-08
影响因子：
1.8
通讯作者：
Saito M
中科院分区：
文献类型：
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作者：
Iwamatsu-Kobayashi Y;Abe S;Fujieda Y;Orimoto A;Kanehira M;Handa K;Venkataiah VS;Zou W;Ishikawa M;Saito M
表面预反应玻璃离子（S-PRG）填料是复合树脂的一种成分，能够释放出对龋齿和牙周病原体具有抗菌活性的金属离子。虽然S-PRG被认为参与口腔疾病的预防，但尚未发表关于其对体内牙周病的预防作用的报告。本研究调查了来自S-PRG的抑制剂（S-PRG抑制剂）是否对结扎诱导的牙周病小鼠模型中诱导的组织破坏具有抑制作用。将27只C57 BL/6小鼠分为3组，每组9只动物，无结扎组（Lig（-））、结扎组（Lig（+）S-PRG（-））和S-PRG结扎组（Lig（+）S-PRG（+））。使用微型计算机断层扫描评价牙槽骨丢失。苏木精-伊红染色观察组织学变化。用Ly 6 G和F4/80免疫组化染色法检测炎性细胞浸润情况。通过飞行时间二次离子质谱法检测金属离子的分布。S-PRG可明显抑制牙槽骨丢失和骨密度。组织学分析显示，S-PRG可减少牙周膜中胶原束的破坏和炎性细胞的浸润。免疫组织化学分析显示，S-PRG抑制剂显著抑制了浸润性中性粒细胞和巨噬细胞的数量。飞行时间二次离子质谱分析显示，Lig（+）S-PRG（+）组中存在的硼离子多于Lig（+）S-PRG（-）组。我们的研究结果表明，S-PRG通过其体内抗炎作用对牙周病中的组织破坏具有预防作用。
The surface pre‐reacted glass ionomer (S‐PRG) filler, a component of composite resin, is capable of releasing metal ions that possess antibacterial activity against caries and periodontal pathogens. Although S‐PRG has been suggested to be involved in oral disease prevention, no reports have been published regarding its preventive effect on periodontal disease in vivo. The present study investigated whether the eluate from S‐PRG (S‐PRG eluate) has a suppressive effect on tissue destruction induced in a mouse model of ligature‐induced periodontal disease. Twenty‐seven C57BL/6 mice were divided into three groups of nine animals each, no ligature group (Lig(−)), ligature group (Lig(+)S‐PRG(−)) and ligature with S‐PRG eluate group (Lig(+)S‐PRG(+)). Alveolar bone loss was evaluated using micro‐computed tomography scanning. Histologic changes were detected by hematoxylin and eosin staining. The infiltration of inflammatory cells was assessed by Ly6G and F4/80 staining immunohistochemically. The distribution of metal ions was detected by time‐of‐flight secondary ion mass spectrometry. S‐PRG eluate clearly inhibited alveolar bone loss and bone density. The histological analysis revealed that S‐PRG eluate reduced destruction of the collagen bundle in the periodontal ligament and the infiltration of inflammatory cells. Immunohistochemical analysis showed that the S‐PRG eluate significantly suppressed the number of infiltrating neutrophils and macrophages. Time‐of‐flight secondary ion mass spectrometry analysis revealed that more boron ions were present in the Lig(+)S‐PRG(+) group than in the Lig(+)S‐PRG(−) group. Our results suggest that the S‐PRG eluate has a preventive effect against tissue destruction in periodontal disease through its anti‐inflammatory effects in vivo.