Isolated heart myocytes: ultrastructural case study technique.
Isolated heart myocytes: ultrastructural case study technique.
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离体心肌细胞：超微结构案例研究技术。
DOI：
10.1111/j.1365-2818.1981.tb00306.x
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发表时间：
1981
影响因子：
2
通讯作者：
Wittenberg,BA
中科院分区：
文献类型：
--
作者：
Robinson,TF;Hayward,BS;Krueger,JW;Sonnenblick,EH;Wittenberg,BA
我们开发了一种方法，用于对从大鼠心室壁酶促分离的心肌细胞进行案例研究，该方法是为检查单层附着培养细胞而开发的程序的简单且廉价的改编。该技术与已发表的对来自潜在异质分离肌细胞群的颗粒或单层的电子显微镜研究的描述明显不同。在这里，我们报告了该方法，我们在受控条件下添加了 0 mmol 和 1 mmol CaCl2，将活状态下的肌节长度和电刺激性与超微结构特征（包括肌丝的相对配置和细胞外壳的完整性）相关联。根据活态、固定态和嵌入态条纹照片直接测定，制备步骤中的收缩程度小于 5%。
We have developed a method for performing case studies of heart muscle cells enzymatically isolated from the ventricular walls of rats that is a simple and inexpensive adaptation of procedures developed for the examination of monolayers of attached, cultured cells. The technique represents a marked departure from published accounts of electron microscopic studies of pellets or monolayers from a population of potentially heterogeneous isolated myocytes. Here we report the method, which we have used under controlled conditions with 0 mmol and 1 mmol added CaCl2, to correlate sarcomere length and electrical stimulatibility in the living state with ultrastructural features that include the relative disposition of myofilaments and the integrity of the cell coat. The degree of shrinkage during the preparative steps is less than 5%, as directly determined from photographs of striations in the living, fixed, and embedded states.