Chemical Characterization and Antioxidant Activity of Mountain Pine (Pinus mugo Turra, Pinaceae) from Republic of Macedonia
Chemical Characterization and Antioxidant Activity of Mountain Pine (Pinus mugo Turra, Pinaceae) from Republic of Macedonia
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DOI：
10.25135/rnp.73.18.02.233
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发表时间：
2019-01-01
影响因子：
1.9
通讯作者：
Kulevanova, Svetlana
中科院分区：
文献类型：
--
作者：
Karapandzova, Marija;Stefkov, Gjoshe;Kulevanova, Svetlana
本文研究了原产于日本的黑松针叶精油和甲醇提取物的化学组成和抗氧化活性。马其顿进行了调查。植物材料收集卡拉季察山在两个季节。采用水蒸气蒸馏法提取挥发油，用GC/FID/MS分析，鉴定出8种主要成分：δ（3）-carene、α-pinene、limonene+beta-phellandrene、germacrene D、trans-carryllene、δ-cadinene、bicyclogermacrene和α-cadinol。采用LC-DAD/ESI-MSn对甲醇提取物中的黄酮类和酚类化合物进行了鉴定。共鉴定出14个化合物，其中酚酸类2个，原花青素类2个，黄酮醇苷类7个，黄酮醇酰化苷类3个。分别采用Folin-Ciocalteau法和氯化铝法测定总酚和总黄酮含量。对于干燥的植物材料，TPC和TFC值分别为11.41-12.17 mgGAE/g和4.65-6.03 mgCE/g。采用DPPH法和TBARS法评价其抗氧化活性。甲醇提取物对DPPH的IC 50值范围为11.58-16.01 mg/mL，对TBARS测定的IC 50值范围为9.41-15.76 mg/mL。对于DPPH和TBARS测定，精油的IC 50值分别为2.51-4.26 mg/mL和2.59-4.14 mg/mL。由于其具有良好的抗氧化活性，因此可以考虑将其作为一种潜在的植物源，用于精油以及黄酮类化合物和其他酚类化合物的分离。此外，还应进一步研究和评价其化学成分和生物活性，为可能的药用、制药和商业利用提供更多的数据。
Chemical composition and antioxidant activity of essential oils and methanol extracts of needles of Pinus mugo native to R. Macedonia were investigated. Plant material was collected on Karadzica Mountain during two seasons. Essential oil was obtained by steam distillation and was analyzed by GC/FID/MS. 8 major constituents were identified: Delta(3)-carene, alpha-pinene, limonene+beta-phellandrene, germacrene D, trans-caryophyllene, delta-cadinene, bicyclogermacrene and alpha-cadinol. Identification of flavonoids and phenolic compounds in methanol extracts was made by LC-DAD/ESI-MSn. 14 compounds were identified (2 phenolic acids, 2 procyanidins, 7 flavonol glycosides and 3 flavonol acylated glycosides). Total phenolic content (TPC) and total flavonoid content (TFC) were measured by Folin-Ciocalteau and aluminum chloride assay, respectively. TPC and TFC values ranged from 11.41-12.17 mgGAE/g and from 4.65-6.03 mgCE/g for dried plant material, respectively. Evaluation of antioxidant activity was made using DPPH and TBARS test. IC50 values for the methanol extract ranged from 11.58-16.01 mg/mL for DPPH and from 9.41-15.76 mg/mL for TBARS assay. IC50 values for the essential oil ranged from 2.51-4.26 mg/mL and 2.59-4.14 mg/mL for DPPH and TBARS assay, respectively. The needles from Macedonian P. mugo could be considered as a potential plant source for isolation of essential oil as well as flavonoids and other phenolics as they exhibit promising antioxidant activity. Additionally, further investigation and evaluation of chemical composition as well as biological activity should be done in order to provide more data for their possible medicinal, pharmaceutical and commercial utilization.