Enhanced axonal growth from fetal human bcl-2 transgenic mouse dopamine neurons transplanted to the adult rat striatum
Enhanced axonal growth from fetal human bcl-2 transgenic mouse dopamine neurons transplanted to the adult rat striatum
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DOI：
10.1016/s0306-4522(01)00098-7
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Isacson, O
中科院分区：
文献类型：
--
作者：
Holm, KH;Cicchetti, F;Isacson, O
移植到成年中枢神经系统的胚胎神经元只能在发育规定的时间内延伸轴突。有某些细胞间因素控制轴突的伸展，其中之一可能是bcl2蛋白的表达。在这项研究中，完全去纹状体多巴胺纤维的MTS接受胚胎第14天过表达人bcl2的小鼠腹侧中脑细胞或对照野生型腹侧中脑细胞。所有大鼠均接受环孢素治疗以防止排斥反应，并对存活的移植物进行细胞存活和多巴胺能纤维生长的分析。结果表明，bcl2过表达并不能提高神经元移植物的存活率。这些结果表明，bcl2过表达可以导致移植的胎儿多巴胺能神经元长得更远，胚胎供体细胞的基因修饰可能会增强它们重新支配神经元靶区的能力。(C)2001年IBRO。爱思唯尔科学有限公司出版。版权所有。
Embryonic neurons transplanted to the adult CNS extend axons only for a developmentally defined period. There are certain intercellular factors that control the axonal extension, one of which may be the expression of the bcl-2 protein. In this study, mts with complete striatal dopamine fiber denervation received embryonic day 14 mouse ventral mesencephalon cells overexpressing human bcl-2 or control wild-type ventral mesencephalon cells. All rats were treated with cyclosporine to prevent rejection and the surviving grafts were analyzed for cell survival and outgrowth of dopaminergic fibers. The results demonstrate that bcl-2 overexpression does not enhance neuronal graft survival. However, the bcl-2 overexpressing neurons had a higher number of dopaminergic fibers that grew longer distances.These results show that overexpression of bcl-2 can result in longer distance axonal growth of transplanted fetal dopaminergic neurons and that genetic modification of embryonic donor cells may enhance their ability to reinnervate a neuronal target territory. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.