The impact of forced joint exercise on lubricin biosynthesis from articular cartilage following ACL transection and intra-articular lubricin's effect in exercised joints following ACL transection
The impact of forced joint exercise on lubricin biosynthesis from articular cartilage following ACL transection and intra-articular lubricin's effect in exercised joints following ACL transection
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DOI：
10.1016/j.joca.2012.04.021
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发表时间：
2012-08-01
影响因子：
7
通讯作者：
Jay, G. D.
中科院分区：
文献类型：
--
作者：
Elsaid, K. A.;Zhang, L.;Jay, G. D.
目的：为了评估急性膝关节损伤后强迫关节运动对润滑素代谢的影响及其与软骨退变的关系，并评估单剂量纯化人润滑素注射液在运动损伤关节中的软骨保护作用。ACLT后3周、ACLT+运动后3周、ACLT后5周、ACLT+运动后5周和ACLT+运动后5周接受关节内磷酸盐缓冲盐水（PBS）或润滑素治疗。从术后1周开始，使用旋转圆筒以6 rpm的速度每天进行30分钟的关节锻炼，每周5天。测定受损关节中软骨润滑素的表达。组织学分析包括番红O/Fast绿色、活化半胱天冬酶-3和润滑素mRNA原位杂交。结果：3周时，运动ACLT关节中润滑素的表达显著低于对照组（P <0.001），运动组关节中润滑素的表达显著低于对照组（P < 0.001）。5周时，ACLT +运动组动物的OARSI评分显著高于ACLT组动物（P < 0.001）。与3周ACLT相比，3周ACLT +运动软骨显示caspase-3染色增加。与ACLT +运动和PBS治疗的ACLT +运动相比，关节内润滑素治疗导致软骨润滑素基因表达的显著增加（P < 0.001）和uCTXII水平的降低（P < 0.05）。关节内注射润滑素可改善运动引起的软骨损伤，并保持浅表区软骨细胞的活力。(C)2012年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: To evaluate the impact of forced joint exercise following acute knee injury on lubricin metabolism and its relationship to cartilage degeneration and to assess chondroprotection of a single-dose purified human lubricin injection in exercised injured joints.Methods: Anterior cruciate ligament transection (ACLT) was performed in rats with six experimental groups; 3-week post-ACLT, 3-week post-ACLT + exercise, 5-week post-ACLT, 5-week post-ACLT + exercise, and 5-week post-ACLT + exercise treated with intra-articular phosphate buffered saline (PBS) or lubricin. Joint exercise was achieved using a rotating cylinder at a speed of 6 rpm for 30 min daily, 5 days a week starting 1 week following surgery. Cartilage lubricin expression in injured joints was determined. Histological analyses included Safranin O/Fast Green, activated caspase-3, and lubricin mRNA in-situ hybridization. Assessment of cartilage damage was performed by osteoarthritis research society international (OARSI) modified Mankin scoring and urinary CTXII (uCTXII) levels.Results: At 3 weeks, lubricin expression in exercised ACLT joints was significantly (P < 0.001) lower compared to ACLT joints. The OARSI scores were significantly (P < 0.001) higher in the ACLT + exercise animals compared to ACLT animals at 5 weeks. Compared to 3-week ACLT, 3-week ACLT + exercise cartilage showed increased caspase-3 staining. Compared to ACLT + exercise and PBS-treated ACLT + exercise, lubricin intra-articular treatment resulted in a significant increase (P < 0.001) in cartilage lubricin gene expression and a reduction (P < 0.05) in uCTXII levels.Conclusion: Joint exercise resulted in decreased lubricin cartilage expression, increased cartilage degeneration and reduced superficial zone chondrocyte viability in the ACLT joint. Intra-articular lubricin administration ameliorated cartilage damage due to exercise and preserved superficial zone chondrocytes' viability. (C) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.