Endoscopic confocal fluorescence microscopy of normal and tumor bearing rat bladder
Endoscopic confocal fluorescence microscopy of normal and tumor bearing rat bladder
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DOI：
10.1097/01.ju.0000164729.36663.8d
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发表时间：
2005-08-01
影响因子：
6.6
通讯作者：
Guillemin, F
中科院分区：
文献类型：
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作者：
D'Hallewin, MA;El Khatib, S;Guillemin, F
目的：探讨内窥镜下纤维共聚焦显微镜在大鼠膀胱模型中应用的可行性，并区分不同的细胞类型。材料和方法：将罗丹明123(分子探针，俄勒冈州尤金)(100亩M)注入5只荷瘤大鼠(AY27)的膀胱中30分钟。5只正常大鼠作为对照。一根Cell-Vizio(TM)共聚焦显微镜纤维被放置在经尿道与正常或转化的膀胱壁接触的地方。结果：由于细胞形态和荧光强度的不同，利用Cell-Vizio(TM)设备可以很容易地识别出大鼠上皮细胞的不同类型(伞状细胞、中间细胞和基底细胞)。由于强烈的荧光信号，无法区分单个AY-27细胞，但整个肿瘤呈现为一个明亮的均匀荧光斑点，周围环绕着细小的炎症细胞。结论：我们在体内报道了在大鼠膀胱内窥镜下进行光纤共聚焦显微镜的可行性。我们将肿瘤与正常上皮区分开来，并在未转化的大鼠膀胱上皮中观察到不同的上皮细胞类型。
Purpose: We evaluated the possibility of performing endoscopic fiber-optic confocal microscopy in a rat bladder model and we distinguished different cell types.Material and Methods: Rhodamine 123 (Molecular Probes, Eugene, Oregon) (100 mu M) was instilled for 30 minutes in 5 tumor bearing rat bladders (AY27). Five normal rats served as controls. A Cell-vizio (TM) confocal microscopy fiber was placed transurethrally in contact with normal or transformed bladder wall. Frozen sections were obtained from the same spots and subjected to conventional fluorescence microscopy and anatomical-pathological analysis.Results: The different cells types present in rat epithelium (umbrella, intermediate and basal cells) could easily be identified with the Cell-Vizio (TM) device due to their differences in morphology and fluorescence intensity. Individual AY-27 cells could not be demarcated due to the strong fluorescence signal but the entire tumor appeared as a brightly homogenous fluorescent blot surrounded by small inflammatory cells.Conclusions: We report the feasibility of endoscopic, in vivo, fiber-optic confocal microscopy in the rat bladder. We distinguished tumors from normal epithelium and visualized the different epithelial cell types in nontransformed rat bladder epithelium.