MOLECULAR-CLONING OF A LIGAND FOR THE FLT3/FLK-2 TYROSINE KINASE RECEPTOR - A PROLIFERATIVE FACTOR FOR PRIMITIVE HEMATOPOIETIC-CELLS
MOLECULAR-CLONING OF A LIGAND FOR THE FLT3/FLK-2 TYROSINE KINASE RECEPTOR - A PROLIFERATIVE FACTOR FOR PRIMITIVE HEMATOPOIETIC-CELLS
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DOI：
10.1016/0092-8674(93)90325-k
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发表时间：
1993-12-17
期刊：
影响因子：
64.5
通讯作者：
BECKMANN, MP
中科院分区：
文献类型：
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作者：
LYMAN, SD;JAMES, L;BECKMANN, MP
使用可溶形式的受体进行鼠flt 3/flk-2酪氨酸激酶受体的配体的克隆以鉴定配体的来源。鼠T细胞系P7 B-0.3A4被鉴定为似乎表达该受体的细胞表面配体。从由这些细胞制备的表达文库中分离cDNA克隆，当转染到细胞中时，所述cDNA克隆能够赋予与可溶形式的flt 3/flk-2受体的结合。该配体的cDNA编码I型跨膜蛋白，其刺激用flt 3/flk-2受体转染的细胞的增殖。可溶形式的配体刺激小鼠骨髓和胎肝细胞以及高度富集造血干细胞和原始未定型祖细胞的人骨髓细胞的限定亚群的增殖。
Cloning of a ligand for the murine flt3/flk-2 tyrosine kinase receptor was undertaken using a soluble form of the receptor to identify a source of ligand. A murine T cell line, P7B-0.3A4, was identified that appeared to express a cell surface ligand for this receptor. A cDNA clone was isolated from an expression library prepared from these cells that was capable, when transfected into cells, of conferring binding to a soluble form of the flt3/flk-2 receptor. The cDNA for this ligand encodes a type I transmembrane protein that stimulates the proliferation of cells transfected with the flt3/flk-2 receptor. A soluble form of the ligand stimulates the proliferation of defined subpopulations of murine bone marrow and fetal liver cells as well as human bone marrow cells that are highly enriched for hematopoietic stem cells and primitive uncommitted progenitor cells.