Prevalence of sulfonamide-resistant bacteria, resistance genes and integron-associated horizontal gene transfer in natural water bodies and soils adjacent to a swine feedlot in northern Taiwan
Prevalence of sulfonamide-resistant bacteria, resistance genes and integron-associated horizontal gene transfer in natural water bodies and soils adjacent to a swine feedlot in northern Taiwan
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DOI：
10.1016/j.jhazmat.2014.02.016
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发表时间：
2014-07-30
影响因子：
13.6
通讯作者：
Ying, Chingwen
中科院分区：
文献类型：
--
作者：
Hsu, Jih-Tay;Chen, Chia-Yang;Ying, Chingwen
抗生素通常用于猪饲料中，用于治疗和预防疾病，以及促进生长。通过废水释放到环境中的抗生素会加速周围环境中耐药细菌和耐药基因的出现。在这项研究中，我们量化了台湾北部一个养猪场及其周边天然水体和土壤的废水中磺胺类化合物、耐药微生物和耐药基因的存在情况。在接收下游和上游的河水中，磺胺水平相似。然而，通过依赖培养和独立的分子方法分析的磺胺耐药细菌和耐药基因在下游的流行率明显高于上游河水样本。条形码焦磷酸测序显示，每个样本中的细菌群落结构高度多样化。然而，废水和下游环境样品中磺胺抗性基因sul1的序列同源性几乎相同(99-100%)。在下游水体和土壤中，Sul1基因在基因上与1类整合子连锁，占主导地位。总之，养猪场废水中磺胺类抗药性基因的增加与磺胺类药物的持续存在无关，可能是周围环境中抗药性基因库的潜在来源。(C)2014爱思唯尔B.V.保留所有权利。
Antibiotics are commonly used in swine feed to treat and prevent disease, as well as to promote growth. Antibiotics released into the environment via wastewater could accelerate the emergence of antibioticresistant bacteria and resistance genes in the surrounding environment. In this study, we quantified the occurrence of sulfonamides, sulfonamide-resistant microorganisms and resistance genes in the wastewater from a swine farm in northern Taiwan and its surrounding natural water bodies and soils. Sulfonamide levels were similar in the receiving downstream and upstream river water. However, the prevalence of sulfonamide-resistant bacteria and resistance genes, as analyzed by cultivation-dependent and -independent molecular approaches, was significantly greater in the downstream compared to the upstream river water samples. Barcoded-pyrosequencing revealed a highly diverse bacterial community structure in each sample. However, the sequence identity of the sulfonamide resistance gene sul1 in the wastewater and downstream environment samples was nearly identical (99-100%). The sul1 gene, which is genetically linked to class 1 integrons, was dominant in the downstream water bodies and soils. In conclusion, the increased prevalence of sulfonamide resistance genes in the wastewater from a swine farm, independent of the persistent presence of sulfonamides, could be a potential source of resistant gene pools in the surrounding environment. (C) 2014 Elsevier B.V. All rights reserved.