Changes in the Content of Glial Fibrillary Acidic Protein in the Frontal Cortex of Rats during Conditioned Active Avoidance Training
Changes in the Content of Glial Fibrillary Acidic Protein in the Frontal Cortex of Rats during Conditioned Active Avoidance Training
复制标题
DOI：
10.1023/a:1026060607740
复制
发表时间：
2003-03-01
期刊：
影响因子：
0.5
通讯作者：
Chorna, V. I.
中科院分区：
文献类型：
--
作者：
Drozdov, O. L.;Chorna, V. I.
本实验研究了条件性主动回避反应（CAAR）训练过程中大鼠额叶新皮层胶质细胞酸性蛋白（GFAP）表达的动态变化。使用固相免疫酶分析，2小时和1，3，7，14，21，和30天后开始训练的动物的额皮质组织的细胞骨架部分中的GFAP含量进行定量估计。结果发现，GFAP在新皮层中的丝状形式的表达的变化是明显明显的，并且具有三相模式：GFAP水平在CAAR训练开始后的第一个小时内显著增加，在7-14天内该指数下降并变得小于初始状态，然后再次上升（在21天后达到最大值）。获得的数据表明，所研究的神经特异性蛋白参与学习和条件记忆痕迹形成的过程。
We studied the dynamics of expression of glial fibrillary acidic protein (GFAP) in the frontal neocortex of rats during training for conditioned active avoidance reaction (CAAR). The GFAP content in a cytoskeletal fraction of the frontal cortex tissue was quantitatively estimated with the use of solid-phase immunoenzyme analysis, 2 h and 1, 3, 7, 14, 21, and 30 days after the beginning of training of the animals. It was found that the changes in expression of the filamentous form of GFAP in the neocortex are clearly pronounced and possess a three-phase pattern: the level of GFAP significantly increased within the first hours after the beginning of CAAR training, within 7-14 days this index decreased and became smaller than that in the initial state, and then again rose (with a maximum after 21 days). The obtained data indicate that the neurospecific protein under study is involved in the processes of learning and formation of conditioned memory engrams.