INTESTINAL SPIROCHETOSIS - MORPHOLOGICAL CHARACTERIZATION AND CULTIVATION OF THE SPIROCHETE BRACHYSPIRA-AALBORGI GEN-NOV, SP-NOV
INTESTINAL SPIROCHETOSIS - MORPHOLOGICAL CHARACTERIZATION AND CULTIVATION OF THE SPIROCHETE BRACHYSPIRA-AALBORGI GEN-NOV, SP-NOV
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DOI：
10.1128/jcm.16.6.1127-1136.1982
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发表时间：
1982-01-01
影响因子：
9.4
通讯作者：
THAYSEN, EH
中科院分区：
文献类型：
--
作者：
HOVINDHOUGEN, K;BIRCHANDERSEN, A;THAYSEN, EH
本文用负染法和螺旋切片法研究了肠道螺旋体病患者直肠活检标本中螺旋体的超微结构。细胞呈S形，末端逐渐变细，2-6 μ m长，波长为2 μ m。在细胞的每一端插入四个鞭毛。最大细胞宽度为约0.2 μ m。螺旋体在胰蛋白胨大豆血琼脂平板上培养。它们是厌氧的，在37 - 38.5 ℃生长，尽管非常缓慢。在5%CO2 - 95%H2的气氛中。可以区分两种类型的殖民地。分离的螺旋体的生长特征和形态不同于先前分离的螺旋体菌株。建议本菌株构成密螺旋体科的一个新属，短螺旋体。模式种为B。aalborgi，其模式株为513 A（NCTC 11492）。
The ultrastructure of spirochetes obtained from rectal biopsies of patients with intestinal spirochetosis was studied by means of negative staining and ultrathin sectioning. The cells were sigmoidal with tapered ends, 2-6 .mu.m long, with a wavelength of 2 .mu.m. Four flagella were inserted at each end of the cells. The maximal cell width was about 0.2 .mu.m. The spirochetes were cultured on tryptose soy blood agar plates. They were anaerobic and grew, although very slowly, at 37-38.5.degree. C in an atmosphere of 5% CO2-95% H2. Two types of colonies could be distinguished. The growth characteristics and the morphology of the isolated spirochetes differ from those of previously isolated spirochetal strains. It is proposed that the present strains constitute a new genus, BRACHYSPIRA, of the family Treponemataceae. The type species is B. aalborgi, the type strain of which is 513A (NCTC 11492).