SEQUENCE VARIABILITY IN THE GENOME-3'-TERMINAL REGION OF BYDV FOR 10 GEOGRAPHICALLY DISTINCT PAV-LIKE ISOLATES OF BARLEY YELLOW DWARF VIRUS - ANALYSIS OF THE ORF6 VARIATION
SEQUENCE VARIABILITY IN THE GENOME-3'-TERMINAL REGION OF BYDV FOR 10 GEOGRAPHICALLY DISTINCT PAV-LIKE ISOLATES OF BARLEY YELLOW DWARF VIRUS - ANALYSIS OF THE ORF6 VARIATION
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DOI：
10.1007/bf01310801
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发表时间：
1994-01-01
影响因子：
2.7
通讯作者：
LAPIERRE, HD
中科院分区：
文献类型：
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作者：
CHALHOUB, BA;KELLY, L;LAPIERRE, HD
对来自三大洲的10个大麦黄矮病毒(BYDV)PAV样分离物的基因组末端3‘端至衣壳读入顺反子的核苷酸序列进行了比较。测序区域的长度从853到864个核苷酸不等，各分离株之间的序列同源性在84%到99%之间。序列变异主要发生在两个位置，一个在ORF6编码区，另一个在基因组3‘端附近。序列同源性分组揭示了三个遗传上不同的PAV分离物簇(A、B和C)。A群由澳大利亚分离株组成，B群由1个加拿大分离株和3个法国分离株组成，C群由法国分离株RG组成。与BYDV-MAV血清型相应基因组3‘末端区域的差异比PAV分离株之间的差异更大。与大豆矮缩病毒3‘端非翻译区序列比较，发现两个核苷酸序列具有相似性，表明该病毒具有共同的祖先。编码能力的研究表明，ORF6在所有已测序的PAV分离株中都存在，但在大小和推导的氨基酸组成上存在差异。然而，大多数核苷酸变化仅限于ORF6密码子的第三个碱基位置这一事实表明，ORF6编码一种功能蛋白质。
Nucleotide sequence of the genome terminal region 3' to the capsidreadthrough cistron were compared for 10 PAV-like isolates of barley yellow dwarf virus (BYDV) from three continents. The sequenced region varied in length from 853 to 864 nucleotides and the extent of sequence homology among the isolates ranged from 84 to 99%. Sequence variations occur mainly in two locations, one in the ORF6 coding region and the other near the genome 3' terminus. Sequence homology grouping reveals three genetically distinct clusters of PAV isolates (A, B and C). Cluster A consists of the Australian isolates, cluster B of one Canadian and three French isolates, and cluster C of the French isolate, RG. Dissimilarities with the corresponding genome-3'- terminal region of the BYDV-MAV serotype were greater than those observed between the PAV isolates alone. Comparison with the sequence of the 3' untranslated region of soybean dwarf virus revealed two stretches of nucleotide similarity, suggesting a common ancestor. Study of the coding ability revealed that the ORF6 is present in all the sequenced PAV isolates but differs in size and deduced amino acids composition. However, the fact that the majority of nucleotide changes are restricted to the third base position of the ORF6-codons suggests that ORF6 codes for a functional protein.