REPLICATION OF ATYPICAL OVINE ROTAVIRUS IN SMALL-INTESTINE AND CELL-CULTURE
REPLICATION OF ATYPICAL OVINE ROTAVIRUS IN SMALL-INTESTINE AND CELL-CULTURE
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DOI：
10.1099/0022-1317-67-3-567
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发表时间：
1986-03-01
影响因子：
3.8
通讯作者：
BANKS, J
中科院分区：
文献类型：
--
作者：
CHASEY, D;BANKS, J
初乳剥夺羔羊实验感染的非典型绵羊轮状病毒分离自然冲刷动物，和自然感染初乳剥夺羔羊，进行了检查免疫荧光和免疫过氧化物酶标记，和电子显微镜。在无菌羔羊中产生针对该病毒的超免疫血清，并用于证明来自感染动物的小肠绒毛上皮细胞中的抗原。扫描和透射电子显微镜从感染动物的组织显示巨大的多核合胞体融合的上皮细胞组成。感染细胞胞浆内含有类似于A组轮状病毒的病毒颗粒的形态和形态发生。接种绵羊非典型轮状病毒的MA 104培养物中的少量感染细胞可以通过免疫荧光检测到，但病毒生长不能通过传代维持。通过薄层电子显微镜观察到病毒颗粒，但其形态和形态发生异常;它们均由单个电子致密壳组成，没有核心，并且与光滑膜而不是粗糙内质网的包膜相关。
Colostrum-deprived lambs experimentally infected with an atypical ovine rotavirus isolated from naturally scouring animals, and a naturally infected colostrum-deprived lamb, were examined by immunofluorescence and immunoperoxidase labelling, and electron microscopy. A hyperimmune serum to the virus was produced in a gnotobiotic lamb and used to demonstrate antigen in the villous epithelial cells of the small intestine from infected animals. Scanning and transmission electron microscopy of tissues from infected animals revealed giant multinucleate syncytia composed of fused epithelial cells. Infected cells contained intracytoplasmic virus particles which resembled group A rotaviruses in morphology and morphogenesis. Small numbers of infected cells in MA104 cultures inoculated with ovine atypical rotavirus could be detected by immunofluorescence but virus growth could not be maintained by passage. Virus particles were seen by thin-section electron microscopy but their morphology and morphogenesis were abnormal; they were each composed of a single electron-dense shell, with no core, and were associated with envelopes of smooth membrane rather than rough endoplasmic reticulum.