Genetic variability of quantitative traits in Drosophila melanogaster (fruit fly) natural populations:: analysis of wild-living flies and of several laboratory generations
Genetic variability of quantitative traits in Drosophila melanogaster (fruit fly) natural populations:: analysis of wild-living flies and of several laboratory generations
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DOI：
10.1046/j.1365-2540.1998.00301.x
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发表时间：
1998-03-01
期刊：
影响因子：
3.8
通讯作者：
David, JR
中科院分区：
文献类型：
--
作者：
Gibert, P;Moreteau, B;David, JR
将野生收集的黑腹果蝇雌性分离在培养瓶中以在实验室中起始同雌系。翅长和身体色素沉着（胸部，腹部节5，6和7和他们的总和）进行了测量，在野生苍蝇和连续的实验室世代。通过野生果蝇和实验室后代之间的亲子回归或通过计算每个实验室世代中品系之间的组内相关性来估计遗传力。翅长的表型变异在自然条件下比在实验室条件下高得多，亲子回归估计的遗传力（0.14）是显著的，但相当低。在实验室世代？组内相关性要高得多，平均为0.34。对于色素沉着特征，自然界的变异性与实验室测量的相似。亲子回归产生显着和高的遗传力值（范围0.40-0.62），除了腹部节段5。组内相关性也显着大于零的所有性状（范围0.22-0.47），包括段5。连续几代实验室的稳定性线显示的稳定性的整体平均值和家庭的连续几代的手段之间的强正相关。跨世代的相关性表明了性状的遗传重复性，在使用同雌株系的实验中应该是有用的。
Wild-collected Drosophila melanogaster females were isolated in culture vials to initiate isofemale lines in the laboratory. Wing length and body pigmentation (thorax, abdominal segments 5, 6 and 7 and their sum) were measured in wild flies and in successive laboratory generations. Heritability was estimated by parent-offspring regression between wild-living flies and laboratory progeny or by calculating intraclass correlation between lines in each laboratory generation. For wing length, phenotypic variability was much higher in nature than in the laboratory, and the estimated heritability with parent-offspring regression (0.14) was significant but quite low. Within laboratory generations? intraclass correlation was much higher, on average 0.34. For pigmentation characteristics, variability in nature was similar to that measured in the laboratory. Parent-offspring regressions produced significant and high heritability values (range 0.40-0.62), except for abdomen segment 5. Intraclass correlations were also significantly greater than zero for all traits (range 0.22-0.47), including segment 5. The stability of the lines over successive laboratory generations was shown by the stability of the overall mean and by a strong positive correlation between family means of successive generations. The correlation across generations demonstrates a genetic repeatability of the trait and should be useful in experiments using isofemale lines.