Exercise-induced increase in serum interleukin-6 in humans is related to muscle damage
Exercise-induced increase in serum interleukin-6 in humans is related to muscle damage
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DOI：
10.1113/jphysiol.1997.sp021972
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发表时间：
1997-03-15
影响因子：
5.5
通讯作者：
Pedersen, BK
中科院分区：
文献类型：
--
作者：
Bruunsgaard, H;Galbo, H;Pedersen, BK
1.本研究旨在验证运动诱导的循环细胞因子水平升高与肌肉损伤相关的假设。九名健康的年轻男性受试者进行了两次高强度的自行车运动试验，间隔两周。第一次试验包括30分钟的正常自行车运动（同心运动），而第二次包括30分钟的制动与反向旋转（偏心运动）。在每次试验中，选择的工作负荷使心率和血液中的儿茶酚胺水平增加相同。血浆肌酸激酶（CK）、谷草转氨酶和谷丙转氨酶仅在离心运动后显著升高（P < 0.05）。离心运动后血清IL-6水平显著升高，并与随后几天的CK浓度显著相关，而向心运动后无显著变化.离心运动后淋巴细胞总浓度显著高于向心运动（P < 0.05）。这主要是由于在偏心试验期间自然杀伤（NK）细胞和CD 8阳性细胞（CD 8+细胞）的募集明显更加明显。然而，在单核细胞的循环浓度方面，两种类型的工作之间没有显着差异。中性粒细胞浓度仅在向心运动后2 h显著升高.高强度离心运动导致血浆IL-6水平较同心运动显著升高，这一发现支持了运动后细胞因子产生与骨骼肌损伤相关的假设。事实上，离心和同心运动之间没有差异，发现在大多数血液单核细胞亚群的血液中的招聘支持的假设，运动诱导的血浆中的儿茶酚胺增加是一个主要的决定因素，这些细胞进入血液的动员。然而，由于离心运动引起NK细胞和CD 8+细胞浓度更明显的增加，肌肉损伤中涉及的因素也可能有助于这些细胞的募集。
1. This study was performed to test the hypothesis that the exercise-induced increase in circulating cytokine levels is associated with muscle damage. Nine healthy young male subjects performed two high-intensity bicycle exercise trials separated by two weeks. The first trial consisted of 30 min of normal bicycle exercise (concentric exercise), whereas the second consisted of 30 min of braking with reversed revolution (eccentric exercise). The work loads were chosen to give the same increases in heart rate and catecholamine levels in the blood during each trial.2. Significant increases (P < 0.05) in plasma concentration of creatine kinase (CK), aspartate aminotransferase and alanine aminotransferase were observed only after the eccentric exercise. Furthermore, the level of interleukin-6 (IL-6) in serum increased significantly after the eccentric exercise and was significantly correlated to CK concentration in the following days, whereas no significant changes were found after the concentric exercise.3. The total concentration of lymphocytes increased significantly (P < 0.05) as a result of eccentric compared with concentric exercise. This was mainly due to a significantly more pronounced recruitment of natural killer (NK) cells and CD8 positive cells (CD8+ cells) during the eccentric trial. However, no significant differences between the two types of work were found in regard to the circulating concentration of monocytes. The concentration of neutrophils was only significantly increased 2 h after the concentric exercise.4. The finding that high-intensity eccentric exercise caused a more pronounced increase in the plasma level of IL-6, compared with concentric exercise, supports the hypothesis that the post-exercise cytokine production is related to skeletal muscle damage. The fact that no differences between eccentric and concentric exercise were found in the recruitment of most blood mononuclear cell subsets to the blood supports the hypothesis that the exercise-induced increase in plasma catecholamines is a major determinant of the mobilization of these cells into the blood. However, as eccentric exercise caused a more pronounced increase in the concentration of NK cells and CD8+ cells, factors involved in muscle damage may also contribute to the recruitment of these cells.