Regeneration of DI particles of virulent and attenuated rabies virus: genome characterization and lack of correlation with virulence phenotype.
Regeneration of DI particles of virulent and attenuated rabies virus: genome characterization and lack of correlation with virulence phenotype.
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强毒力和减毒狂犬病病毒 DI 颗粒的再生：基因组特征以及与毒力表型缺乏相关性。
DOI：
10.1099/0022-1317-51-1-69
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Clark,HF
中科院分区：
文献类型：
--
作者：
Wunner,WH;Clark,HF
两株固定的狂犬病病毒进行了检查，他们的能力，再生缺陷的干扰（DI）颗粒和DI颗粒生产与表达的毒力可能的相关性。减毒ERA菌株（ERAp）的空斑纯化储备液，其特征是在i.c.接种的成年小鼠中引起自身干扰性死亡反应，是以高作案动机连续死亡的在BHK-21细胞中。到第六次传代时，DI颗粒再生，其沉降速度和DI/RNA大小对应于亲本储备病毒产生的三种DI颗粒大小中最小的一种。在15个连续的高或低m.o.i.期间，在体内未检测到ERA DI颗粒的再生。传代感染的新生小鼠脑，虽然传代的病毒始终引起了自动干扰型死亡反应时，在成年小鼠中进行测定。与亲本Flury HEP储备病毒产生的DI颗粒的一条带相比，减毒Flury HEPpp株在BHK-21或鼠神经母细胞瘤C1300克隆NA细胞中连续传代期间再生了多达三种独特大小类别的DI颗粒。BHK-21细胞适应的Flury HEPpp病毒在NA细胞中连续传代两次后以高浓度接种时未能杀死成年小鼠。然而，病毒变得完全有毒，并且再生的DI颗粒的单一条带是可见的。在NA细胞中第三次连续传代后，可见缺陷颗粒的额外条带。单链RNA与mol.重量从第一个待再生的DI颗粒群中提取出0.62 × 106的DNA。这对应于摩尔。重量亲本减毒Flury HEP病毒的DI/ssRNA特征。然而，在亲本型DI/RNA中，除了ssRNA之外，还可以分离部分dsRNA。在来自强毒NA细胞繁殖的Flury HEPpp病毒的再生DI颗粒中不能检测到双链RNA。这些结果表明，固定狂犬病毒的毒力表型不取决于DI颗粒的存在或不存在。
Two strains of fixed rabies virus were examined for their ability to regenerate defective interfering (DI) particles and for possible correlation of DI particle production with the expression of virulence. A plaque-purified stock of the attenuated ERA strain (ERApp), which characteristically caused an auto-interfering death response in adult mice inoculated i.c., was serially passed at a high m.o.i. in BHK-21 cells. By the sixth passage, DI particles were regenerated that corresponded in sedimentation velocity and DI/RNA size to the smallest of three sizes of DI particles produced by the parental stock virus. The regeneration of ERA DI particlesin vivowas not detected during 15 serial high or low m.o.i. passages of infected newborn mouse brain, though the passaged virus consistently elicited an auto-interfering-type death response when assayed in adult mice. The attenuated Flury HEPppstrain regenerated up to three unique size classes of DI particles during serial passage in BHK-21 or murine neuroblastoma C1300 clone NA cells compared with the one band of DI particles produced by the parental Flury HEP stock virus. The BHK-21 cell-adapted Flury HEPppvirus failed to kill adult mice when inoculated at high concentrations after two serial passages in NA cells. However, the virus became fully virulent and a single band of regenerated DI particles was visible. Additional bands of defective particles were visible following the third serial passage in NA cells. Single-stranded RNA with a mol. wt. of 0.62 × 106was extracted from the first DI particle population to be regenerated. This corresponded in mol. wt. to the DI/ssRNA characteristic of the parental attenuated Flury HEP virus. However, in the parental type DI/RNA, partially dsRNA could be isolated in addition to ssRNA. Double-stranded RNA could not be detected in the regenerated DI particles derived from the virulent NA cell-propagated Flury HEPppvirus. These results suggest that the virulence phenotype of fixed rabies viruses does not depend on the presence or absence of DI particles.