Intestinal colonization by vanA- or vanB2-containing enterococcal isolates of healthy animals in Spain.
Intestinal colonization by vanA- or vanB2-containing enterococcal isolates of healthy animals in Spain.
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西班牙健康动物体内含有 vanA 或 vanB2 的肠球菌分离株在肠道定植。
DOI：
10.1089/107662903322541892
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发表时间：
2003
影响因子：
2.6
通讯作者：
M. Zarazaga
中科院分区：
文献类型：
--
作者：
C. Torres;C. Tenorio;A. Portillo;Maite García;C. Martínez;R. del Campo;F. Ruiz‐Larrea;M. Zarazaga
对1998年在西班牙拉里奥哈回收的健康动物（66头猪，22只宠物）的粪便样本进行了万古霉素耐药肠球菌定植分析。通过PCR和测序分析万古霉素耐药机制。66份猪和宠物样品中检出含钒肠球菌3份（4.5%），22份中检出含钒肠球菌5份（22.7%）。8株vanA分离株（7株为粪肠球菌，1株为粪肠球菌）共检测到7种不相关的脉冲场凝胶电泳图谱。8株vanA肠球菌均检出tet(M)基因，6株和3株分别检出erm(B)和aac（6’）-Ie-aph（2’）-Ia基因。在猪和宠物样品中分别有3%和4.5%的猪和宠物样品中发现含有vanc1的肠球菌（鸡肠球菌）定植。从猪粪中分离出一株鸡大肠杆菌，鉴定出aac（6′）-Ie-aph（2′）-Ia、ant（6′）-Ia、aph（3′）-IIIa、erm(B)和tet(M)基因。在健康猪的粪便样本中检测到1株含vanb2的hirae菌株。在该分离物中，经特异性pcr和测序证实，vanB2基因簇被整合到tn5382样元件中。在该分离物中还检测到tet(M)和erm(B)基因。这是首次在动物和海地大肠杆菌中发现含vanb2肠球菌的报告。
Fecal samples of healthy animals (66 pigs, 22 pets) recovered during 1998 in La Rioja, Spain, were analyzed for vancomycin-resistant enterococci colonization. Vancomycin resistance mechanisms were analyzed by PCR and sequencing. vanA-containing enterococci were detected in 3 of 66 samples (4.5%) and 5 of 22 samples (22.7%) of the pig and pet samples, respectively. Seven unrelated pulsed-field gel electrophoresis (PFGE) patterns were detected among the 8 vanA isolates (7 Enterococcus faecium, 1 E. faecalis). The tet(M) gene was present in all eight vanA enterococcal isolates, while the erm(B) and aac(6')-Ie-aph(2")-Ia genes were detected in 6 and 3 isolates, respectively. Colonization by vanC-1-containing enterococci (E. gallinarum) was demonstrated in 3% and 4.5% of the pig and pet samples. The aac(6')-Ie-aph(2")-Ia, ant(6)-Ia, aph(3')-IIIa, erm(B) and tet(M) genes were identified in one of the E. gallinarum isolates from a pig fecal sample. One vanB2-containing E. hirae strain was detected in the fecal sample of a healthy pig. In this isolate, the vanB2 gene cluster was integrated into the Tn5382-like element, as demonstrated by specific PCRs and sequencing. The tet(M) and erm(B) genes were also detected in this isolate. This is the first report in which a vanB2-containing enterococci is detected in animals and in E. hirae.