Comparative analysis of iTRAQ-based proteome profiles of Schistosoma japonicum female worms coming from single-sex infections and bisexual for infections
Comparative analysis of iTRAQ-based proteome profiles of Schistosoma japonicum female worms coming from single-sex infections and bisexual for infections
复制标题
基于 iTRAQ 的日本血吸虫雌虫单性感染和双性感染的蛋白质组谱比较分析
DOI：
10.1016/j.jprot.2019.103597
复制
发表时间：
2020-02-20
影响因子：
3.3
通讯作者：
Jin, Yamei
中科院分区：
文献类型：
--
作者：
Li, Xiaochun;Qiao, Hongbin;Jin, Yamei
在血吸虫病中，由两性感染的成熟雌性寄生蠕虫（FMS）产生的卵是对宿主造成病理损害和传播疾病的主要原因。单性别感染的雌性蠕虫（FSS）不能完全发育到性成熟或产生正常的卵子。本研究采用同量异位素相对和绝对定量标记（iTRAQ）联用LC-MS/MS技术对日本血吸虫FSS和FMS蛋白质组进行了研究。共鉴定出1477个在FSS和FMS之间差异表达的蛋白质（倍数变化> 1.2，P <0.05）。生物信息学分析表明，FMS表达的蛋白质主要与蛋壳合成、核糖体合成、蛋白质折叠、细胞解毒以及蛋白质代谢和葡萄糖代谢等生物合成过程相关，而FSS表达的蛋白质主要与运动和氧化磷酸化相关。我们的鉴定和FMS和FSS之间的差异表达蛋白质的分析提供了新的见解，以阐明雌性蠕虫性成熟和reproduction.Significance的分子生物学机制：雌性血吸虫必须保持恒定的配对与男性蠕虫的生殖器官分化的接触。成熟的雌蠕虫可产生感染性虫卵，对宿主造成严重的病理损害和传播疾病。未配对的雌性蠕虫仍然很小，性不成熟;它们不能正常产卵。本研究采用iTRAQ-联用LC-MS/MS技术对日本血吸虫单性别感染雌蠕虫（FSS）和双性感染成熟雌蠕虫（FMS）的全蛋白质组进行了研究。对1477个差异表达蛋白进行了分析。进一步研究DEPs在雌虫性成熟和生殖发育中的作用，可为探讨雌虫的成熟和产卵提供理论依据。
In schistosomiasis, eggs produced by bisexual infected mature female schistosome worms (FMS) are the main cause of pathological damage to the host and the dissemination of the disease. Single-sex infected female worms (FSS) cannot completely develop to sexual maturity or produce normal eggs. In this study, isobaric tags for relative and absolute quantitation (iTRAQ)-coupled LC-MS/MS was used to explore the proteome of FSS and FMS of Schistosoma japonicwn. A total of 1477 differentially expressed proteins (fold change > 1.2, P < .05) between FSS and FMS were identified. Bioinformatics analysis indicated that FMS expressed more proteins related to biosynthetic processes, such as eggshell synthesis, ribosomal synthesis, protein folding, cellular detoxification, and metabolic processes such as protein metabolism and glucose metabolism, whereas more proteins related to locomotion and oxidative phosphorylation were expressed in FSS. Our identification and analysis of differentially expressed proteins between FMS and FSS provides new insights to elucidate the molecular biological mechanisms of female worm sexual maturation and reproduction.Significance: Female Schistosome worms must maintain constant pairing contact with male worms for differentiation of their reproductive organs. Mature female worms can produce infectious eggs, cause serious pathological damage to the host and the dissemination of the disease. Unpaired female worms remain small and sexually immature; they do not spawn normally. In this study, iTRAQ-coupled LC-MS/MS was used to explore the whole proteome of single-sex infected female worms (FSS) and bisexual infected mature female worms (FMS) of Schistosoma japonicum. 1477 differentially expressed proteins (DEPs) between FSS and FMS were identified and analyzed. Further research on DEPs' functions in schistosome sexual maturation and reproductive development might provide theoretical bases to explore female maturation and spawning.