Not all muscles meet the same fate when they die
Not all muscles meet the same fate when they die
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DOI：
10.1006/cbir.2000.0669
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发表时间：
2001-01-01
影响因子：
3.9
通讯作者：
Schwartz, LM
中科院分区：
文献类型：
--
作者：
Jones, MEE;Schwartz, LM
在动物中，通常有两种常见的细胞死亡模式：坏死和凋亡。前者是一个被动的过程，表现为细胞肿胀和溶解，而后者涉及细胞收缩和基因介导的、依赖于ATP的过程。与细胞死亡的近端原因无关，细胞身体几乎总是被吞噬细胞移走。然而，这并不是所有细胞都通用的，因为在昆虫的一些骨骼肌程序性死亡过程中，吞噬细胞并没有被注意到。为了进一步探索这一点，我们使用了各种解剖学方法来研究六分蛾节间肌肉(ISM)的死亡。ISM是在成虫羽化后的30小时内死亡的巨细胞。未检测到血细胞或其他与肌膜相关的细胞。未能检测到巨噬细胞并不是由于技术限制，因为免疫组织化学和功能研究表明，它们存在于血淋巴中。没有吞噬作用来清除ISM身体表明，破坏细胞所需的所有生化机制都驻留在ISM本身。这与分析表明Manduca a没有足够数量的巨噬细胞来消费ISM是一致的。鉴于昆虫没有适应性免疫，使用完全细胞自主过程的能力可能是一种发育选择，而脊椎动物不能利用这种选择。(C)2001年学术出版社。
Two general patterns of cell death are usually described in animals: necrosis and apoptosis. The former is a passive process that displays cellular swelling and lysis, while the latter involves cellular shrinkage and gene-mediated, ATP-dependent processes. Independent of the proximal cause of cell death, cell corpses are almost always removed by phagocytic cells. This is far from universal for all cells however, since phagocytic cells have not been noted during the programmed death of some skeletal muscles in insects. To further explore this, we used a variety of anatomical methods to examine the death of the intersegmental muscles (ISMs) of the moth Manduca sexta. The ISMs are giant cells that die during the 30 h following adult emergence. At no stage examined were hemocytes or other cells associated with the sarcolemma. The failure to detect macrophages was not due to technical limitations since immunohistochemical and functional studies demonstrate their presence in the hemolymph. The absence of phagocytosis to remove ISM corpses suggests that all of the biochemical machinery required for cellular destruction is resident within the ISMs themselves. This is consistent with analysis suggesting that Manduca a does not possess sufficient numbers of macrophages to consume the ISMs. Given that insects do not have adaptive immunity, the ability to use a completely cell autonomous process may be a developmental option that cannot be exploited in vertebrates. (C) 2001 Academic Press.