Discrete regions of the sensor protein virA determine the strain-specific ability of Agrobacterium to agroinfect maize.
Discrete regions of the sensor protein virA determine the strain-specific ability of Agrobacterium to agroinfect maize.
复制标题
传感器蛋白 virA 的离散区域决定了农杆菌农杆菌感染玉米的菌株特异性能力。
DOI：
10.1094/mpmi.1997.10.2.221
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Nester,EW
中科院分区：
文献类型：
--
作者：
Heath,JD;Boulton,MI;Raineri,DM;Doty,SL;Mushegian,AR;Charles,TC;Davies,JW;Nester,EW
农杆菌对转化玉米的侵染能力主要取决于virA基因座，virA基因的诱导是影响玉米侵染的主要因素。本研究利用农杆菌侵染技术，进一步探讨了与病毒诱导相关的细菌因子在玉米侵染中的作用。Ti质粒和病毒A源是决定菌株侵染玉米能力的重要因素，单糖结合蛋白ChvE是玉米农杆菌侵染所必需的。来自农杆菌感染感受态菌株C58的VirAC 58的接头结构域足以将非农杆菌感染菌株A348的VirAA 6转化为农杆菌感染感受态。VirAC 58的周质结构域也能够赋予VirAA 6中等水平的农杆菌感染能力。此外，当从其同源Ti质粒背景中取出并置于pTiC58背景中时，来自A348的VirAA 6蛋白是农杆菌感染感受态的。假设并检查了pTiA6编码的VirAA6特异性抑制剂的存在。
The ability ofAgrobacteriumstrains to infect transformation-recalcitrant maize plants has been shown to be determined mainly by thevirAlocus, implicatingvirgene induction as the major factor influencing maize infection. In this report, we further explore the roles ofvirinduction-associated bacterial factors in maize infection using the technique of agroinfection. The Ti plasmid andvirAsource are shown to be important in determining the ability of a strain to infect maize, and the monosaccharide binding protein ChvE is absolutely required for maize agroinfection. The linker domain of VirAC58from an agroinfection-competent strain, C58, is sufficient to convert VirAA6of a nonagroinfecting strain, A348, to agroinfection competence. The periplasmic domain of VirAC58is also able to confer a moderate level of agroinfection competence to VirAA6. In addition, the VirAA6protein from A348 is agroinfection competent when removed from its cognate Ti plasmid background and placed in a pTiC58 background. The presence of a pTiA6-encoded, VirAA6-specific inhibitor is hypothesized and examined.