ULTRASTRUCTURAL CHANGES OF CUPRIZONE ENCEPHALOPATHY IN MICE
ULTRASTRUCTURAL CHANGES OF CUPRIZONE ENCEPHALOPATHY IN MICE
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DOI：
10.1016/0041-008x(71)90235-3
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发表时间：
1971-01-01
影响因子：
3.8
通讯作者：
WELSER, JR
中科院分区：
文献类型：
--
作者：
HEMM, RD;CARLTON, WW;WELSER, JR
本文报道了慢性喂饲铜腙所致小鼠脑损伤的小脑延髓白色质超微结构特点。给小鼠喂食含0.5%铜腙的饲料8周，并在麻醉下用6%缓冲戊二醛灌注。将小脑脚的小块后固定在1%磷酸盐缓冲的四氧化锇中，并包埋在Epon 812中。薄切片用醋酸双氧铀和柠檬酸铅染色，并用RCA-EMU-3f电子显微镜检查。在星形胶质细胞、少突胶质细胞、轴浆、髓鞘和细胞外间隙中观察到超微结构改变。部分星形胶质细胞胞浆呈淡黄色，部分星形胶质细胞胞浆内空泡、纤维样增生灶和空泡样胞浆透明，少突胶质细胞胞浆内出现髓鞘样结构、空泡、致密体和粗糙面内质网增生。组织学空泡化显然是在电子显微镜照片中观察到的髓鞘内和神经胶质空泡化的结果。细胞外间隙中很少出现水肿液。在某些轴突的轴质中观察到营养不良的变化，包括致密体和囊泡的积累。偶尔，增殖的中轴突被发现，当存在时，它取代轴质。
The ultrastructural features of the encephalopathy induced by chronic feeding of cuprizone in the white matter of the cerebellar medulla of mice were characterized. Mice were fed a diet of 0.5% cuprizone for 8 wk and, under anesthesia, were perfused with 6% buffered glutaraldehyde. Small pieces of the cerebellar peduncles were post-fixed in 1% phosphate-buffered osmium tetroxide and embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate and examined with the RCA-EMU-3f electron microscope. Ultrastructural alterations were observed in the astrocytes, oligodendrocytes, axoplasm, myelin, and extracellular space. Some astrocytes had a pale electron lucent cytoplasm, while in other astrocytes, vacuoles, foci of proliferated fibrils, and focal cytoplasmic clearings were found. Myelin figures, vacuoles, structured dense bodies, and proliferated profiles of rough-surfaced endoplasmic reticulum occurred in the cytoplasm of oligodendrocytes. The histologic vacuolation was apparently the result of intramyelinic and glial vacuolation observed in the electron micrographs. Edemia fluid occurred infrequently in the extracellular space. Dystrophic changes were observed in the axoplasm of some axons and consisted of the accumulation of dense bodies and vesicles. Occasionally, proliferation of the mesaxon was found, and when present, it displaced the axoplasm.