Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae
Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae
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DOI：
10.1371/journal.pone.0213309
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发表时间：
2019-03-14
期刊：
影响因子：
3.7
通讯作者：
Matsumoto-Nakano, Michiyo
中科院分区：
文献类型：
--
作者：
Inaba, Hiroaki;Nomura, Ryota;Matsumoto-Nakano, Michiyo
古兰氏卟啉单胞菌是一种动物牙周病原体，根据其编码fimA基因的多样性可将菌毛分为3种基因型（A-C）。越来越多的证据表明，与其他类型的菌株相比，具有C型菌毛的古拉疟原虫菌株毒性更强。这些生物粘附和侵袭牙龈上皮细胞的能力还有待研究。在感染100次、感染时间为90 min时，gulae的黏附和侵袭程度最高。古古假单胞菌C型和部分B型侵染牙龈上皮细胞的水平显著高于其他菌株，侵染效率与II型牙龈假单胞菌相当。细胞松弛素D和叠氮化钠可抑制古拉菌对牙龈上皮细胞的粘附和侵袭，说明这些活性需要肌动蛋白聚合和能量代谢。staurosporine可以阻断牙龈上皮细胞内的侵袭，而这些抑制剂对粘附的影响很小，而nocodazole和cycloheximide对粘附和侵袭的影响都可以忽略不计。古拉菌的蛋白酶对牙龈上皮细胞的粘附和侵袭是必需的，而其DNA、RNA和蛋白质的合成则不是必需的。此外，α 5 β 1整合素抗体可显著抑制古拉菌的粘附和侵袭。这是第一个描述古拉菌粘附和侵袭人类牙龈上皮细胞的报道。
Porphyromonas gulae, an animal periodontal pathogen, possess fimbriae classified into three genotypes (A-C) based on the diversity of fimA genes encoding FimA. Accumulating evidence suggests that P. gulae strains with type C fimbriae are more virulent as compared to those with other types. The ability of these organisms to adhere to and invade gingival epithelial cells has yet to be examined. P. gulae showed the greatest levels of adhesion and invasion at a multiplicity of infection of 100 for 90 min. P. gulae type C and some type B strains invaded gingival epithelial cells at significantly greater levels than the other strains, at the same level of efficiency as P. gingivalis with type II fimbriae. Adhesion and invasion of gingival epithelial cells by P. gulae were inhibited by cytochalasin D and sodium azide, indicating the requirements of actin polymerization and energy metabolism for those activities. Invasion within gingival epithelial cells was blocked by staurosporine, whereas those inhibitors showed little effects on adhesion, while nocodazole and cycloheximide had negligible effects on either adhesion or invasion. P. gulae proteases were found to be essential for adhesion and invasion of gingival epithelial cells, while its DNA and RNA, and protein synthesis were unnecessary for those activities. Additionally, alpha 5 beta 1 integrin antibodies significantly inhibited adhesion and invasion by P. gulae. This is the first report to characterize P. gulae adhesion and invasion of human gingival epithelial cells.