Dietary fish oil reduces skeletal muscle oxygen consumption, provides fatigue resistance and improves contractile recovery in the rat in vivo hindlimb
Dietary fish oil reduces skeletal muscle oxygen consumption, provides fatigue resistance and improves contractile recovery in the rat in vivo hindlimb
复制标题
膳食鱼油可降低骨骼肌耗氧量，提供抗疲劳性并改善大鼠体内后肢的收缩恢复
DOI：
10.1017/s0007114510002928
复制
发表时间：
2010
影响因子：
3.6
通讯作者：
P. McLennan
中科院分区：
文献类型：
--
作者：
G. Peoples;P. McLennan
膳食鱼油可调节骨骼肌膜脂肪酸组成。类似的心膜成分变化可调节心肌耗氧量，提高机械性能。采用大鼠体内自体后肢灌注，研究膜成分对骨骼肌功能的影响。雄性Wistar大鼠分别饲喂饱和脂肪（SF）、n-6 PUFA（富含亚油酸）或n-3 PUFA（鱼油）饲料8周。通过坐骨神经（1 Hz, 6-12 V, 0.05 ms）刺激动物自血灌注的后肢骨骼肌收缩，每10分钟重复一次。饲粮中添加n-3 PUFA显著提高了红、白骨骼肌膜中22:6n-3 DHA和总n-3 PUFA，显著降低了n-6:n-3 PUFA比值（P < 0.05）。但n-3 PUFA组在所有收缩回合中保持较大的收缩张力，休息时恢复较好，在最终收缩回合中产生较大的收缩张力（P < 0.05）。与SF组和n-6 PUFA组相比，n-3 PUFA组收缩时后肢耗氧量显著降低，O2效率指数显著提高（P < 0.05）。SF组恢复时静息耗氧量增加（P < 0.05），而n-3 PUFA组无变化。鱼油喂养后的n-3 PUFA DHA膜掺入与肌肉耗氧效率的提高和肌肉抗疲劳能力的增强有关。
Dietary fish oil modulates skeletal muscle membrane fatty acid composition. Similar changes in heart membrane composition modulate myocardial oxygen consumption and enhance mechanical performance. The rat in vivo autologous perfused hindlimb was used to investigate the influence of membrane composition on skeletal muscle function. Male Wistar rats were fed either saturated fat (SF), n-6 PUFA (linoleic acid rich) or n-3 PUFA (fish oil) diets for 8 weeks. Hindlimb skeletal muscle perfused using the animal's own blood was stimulated via the sciatic nerve (1 Hz, 6-12 V, 0·05 ms) to contract in repeated 10 min bouts. The n-3 PUFA diet markedly increased 22 : 6n-3 DHA, total n-3 PUFA and decreased the n-6:n-3 PUFA ratio (P < 0·05) in red and white skeletal muscle membranes. There was no difference in initial twitch tension but the n-3 PUFA group maintained greater twitch tension within all contraction bouts and recovered better during rest to produce greater twitch tension throughout the final contraction bout (P < 0·05). Hindlimb oxygen consumption during contraction was significantly lower in the n-3 PUFA group compared with the SF group, producing a significantly higher O2 efficiency index compared with both SF and n-6 PUFA groups (P < 0·05). Resting oxygen consumption was increased in recovery in the SF group (P < 0·05) but did not change in the n-3 PUFA group. Membrane incorporation of n-3 PUFA DHA following fish oil feeding was associated with increased efficiency of muscle O2 consumption and promoted resistance to muscle fatigue.