ESTABLISHMENT OF A HIGH PRODUCTION SYSTEM FOR AIDS RETROVIRUSES WITH A HUMAN T-LEUKEMIC CELL-LINE MOLT-4
ESTABLISHMENT OF A HIGH PRODUCTION SYSTEM FOR AIDS RETROVIRUSES WITH A HUMAN T-LEUKEMIC CELL-LINE MOLT-4
复制标题
DOI：
10.1016/0304-3835(86)90054-6
复制
发表时间：
1986-03-01
期刊：
影响因子：
9.7
通讯作者：
YAMAMOTO, N
中科院分区：
文献类型：
--
作者：
KOYANAGI, Y;HARADA, S;YAMAMOTO, N
为持续高效地生产艾滋病病毒，建立了一套细胞培养系统。HTLV-III和LAV感染人T细胞系Molt-4后，细胞持续生长并持续产生大量病毒。用逆转录酶活性或新建立的活性病毒生物定量方法来评估病毒产量。该细胞系统的病毒载量远高于H9细胞系统。这一程序使我们能够首先在同一细胞系中直接比较两个病毒株HTLV-III和LAV。培养系统的建立，使艾滋病逆转录病毒的高效生产，为从艾滋病患者体内分离病毒和进行更基础的研究提供了有用的工具，如病毒导致各种恶性肿瘤发生的免疫破坏机制。
A cell culture system was developed for the continuous and efficient production of acquired immune of deficiency syndrome (AIDS) retrovirus. After infection of a human T-cell line Molt-4 with HTLV-III and LAV the cells grow permanently and produce large amounts of virus continuously. The yields of production of virus were assessed either with reverse transcriptase activity or a newly established biological quantitation assay of active virus. The amounts of virus with this cell system were much higher than those of the H9 cell system. This procedure enabled us first to compare the two viral isolates HTLV-III and LAV directly in the same cell line. Establishment of the culture system, allowing efficient production of AIDS retroviruses, provides a useful tool for the isolation of the virus from patients with AIDS and for more basic research, such as the mechanisms of immune destruction caused by the virus leading to the occurrence of various malignancies.