Solid-phase extraction of 11-Nor-Δ9-tetrahydrocannabinol-9-carboxylic acid from urine drug-testing specimens with the Cerex PolyCrom-THC™ column
Solid-phase extraction of 11-Nor-Δ9-tetrahydrocannabinol-9-carboxylic acid from urine drug-testing specimens with the Cerex PolyCrom-THC™ column
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DOI：
10.1093/jat/24.4.245
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发表时间：
2000-05-01
影响因子：
2.5
通讯作者：
Urry, FM
中科院分区：
文献类型：
--
作者：
Crockett, DK;Nelson, G;Urry, FM
通过气相色谱-质谱 (GC-MS) 确认药物滥用是药物检测实验室使用的最耗时的过程。测试结果的成本效益和有竞争力的周转时间需要快速、高效和可靠的提取方法。我们应用 Cerex Polycrom-THC 固相萃取 (SPE) 柱从尿液中萃取 11-nor-Δ9-四氢大麻酚羧酸 (9-THCA)。该柱使用阴离子交换二乙烯基苯共聚物，在用碱水解 THC-葡萄糖苷酸后不需要调节 pH 值，而许多其他 SPE 柱则需要调节 pH 值。对于尿液，无需对 SPE 柱进行预处理。萃取后，用N-甲基-N-(三甲基甲硅烷基)三氟乙酰胺进行三甲基甲硅烷基化衍生化。由于不需要加热孵化，这进一步提高了效率。进行了与现有液-液萃取相关的方法。使用 Finnigan Voyager GC-MS 进行分析，每次进样运行时间为 2.5 分钟。使用 9-THCA-d9 氘代内标，测定在 2 至 2000 ng/mL 范围内呈线性。15 ng/mL 截止浓度下的总精度为 4.4%。还使用两种常见的药物测试掺假物 Klear™ 和 Urine Luck™ 评估了 Cerex 色谱柱的干扰情况。与现有的液-液方法相比，观察到的干扰要少得多。
Confirmation of drugs of abuse by gas chromatography-mass spectrometry (GC-MS) is the most time-consuming process used by drug-testing laboratories. Cost effectiveness and competitive turnaround times for testing results demand fast, efficient, and reliable extraction methods. We applied the Cerex Polycrom-THC solid-phase extraction (SPE) column to the extraction of 11-nor-Δ9-tetrahydrocannabinol carboxylic acid (9-THCA) from urine. This column uses an anion exchange divinyl-benzene copolymer, which requires no pH adjustment after hydrolysis of the THC-glucuronide with base, as is necessary with many other SPE columns. With urine, no preconditioning of the SPE column was necessary. After extraction, trimethylsilyation derivatization was performed withN-methyl-N-(trimethylsilyl) trifluoroacetamide. This further improved efficiency because no heated incubation was required. A method correlation to an existing liquid-liquid extraction was performed. Analysis was on a Finnigan Voyager GC-MS with a 2.5-min run time per injection. Using 9-THCA-d9deuterated internal standard, the assay was linear from 2 to 2000 ng/mL Total precision at the 15-ng/mL cutoff concentration was 4.4%. The Cerex column was also evaluated for interference using two common drug-test adulterants, Klear™and Urine Luck™. Considerably less interference was observed when compared to an existing liquid-liquid method.