Assessing enzyme activities using stable isotope Labeling and mass spectrometry
Assessing enzyme activities using stable isotope Labeling and mass spectrometry
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DOI：
10.1074/mcp.m700057-mcp200
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发表时间：
2007-10-01
影响因子：
7
通讯作者：
Gygi, Steven P.
中科院分区：
文献类型：
--
作者：
Everley, Patrick A.;Gartner, Carlos A.;Gygi, Steven P.
基于活性的蛋白质图谱已经成为标记、浓缩和评估复杂混合物中蛋白质活性的一项有价值的技术。这主要是通过两个步骤的识别和量化过程完成的。在这里，我们展示了一种高度定量和简化的方法，称为酶的捕获和释放活性分析(CAPE)，它将这一过程减少到一个步骤。此外，CAPE方法能够检测到替代的基于质谱学的技术可能遗漏的微小定量变化。
Activity-based protein profiling has emerged as a valuable technology for labeling, enriching, and assessing protein activities from complex mixtures. This is primarily accomplished via a two-step identification and quantification process. Here we show a highly quantitative and streamlined method, termed catch-and-release activity profiling of enzymes ( CAPE), which reduces this procedure to a single step. Furthermore the CAPE approach has the ability to detect small quantitative changes that may have been missed by alternative mass spectrometry-based techniques.