Incorporation of the yeast mitochondrial ribosomal protein Mrp2 into ribosomal subunits requires the mitochondrially encoded Var1 protein.
Incorporation of the yeast mitochondrial ribosomal protein Mrp2 into ribosomal subunits requires the mitochondrially encoded Var1 protein.
复制标题
将酵母线粒体核糖体蛋白 Mrp2 掺入核糖体亚基需要线粒体编码的 Var1 蛋白。
DOI：
10.1007/bf00293206
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ellis,SR
中科院分区：
文献类型：
--
作者：
Davis,SC;Ellis,SR
Mrp2是酿酒酵母（Saccharomycescerevisiae）线粒体核糖体小亚基的蛋白组分。我们研究了Mrp2在缺乏线粒体DNA的酵母突变体中的表达，发现Mrp2的稳态水平相对于野生型显著降低。这些数据表明，Mrp2的积累取决于一个或多个线粒体基因产物的表达。对S.该基因编码核糖体小亚基的两种成分，15S rRNA和Var1蛋白，这两种成分都是成熟37S亚基形成所必需的。一些研究表明，在缺乏Var1的情况下，不完整的亚基积累，缺乏有限数量的核糖体蛋白。在这里，我们表明，Mrp2是一个缺乏Var1亚基的蛋白质，表明Var1在Mrp2纳入线粒体核糖体亚基中起着重要的作用。
Mrp2 is a protein component of the small subunit of mitochondrial ribosomes in the yeastSaccharomyces cerevisiae. We have examined the expression of Mrp2 in yeast mutants lacking mitochondrial DNA and found that the steady-state level of Mrp2 is dramatically decreased relative to wild type. These data suggest that the accumulation of Mrp2 depends on the expression of one or more mitochondrial gene products. The mitochondrial genome ofS. cerevisiaeencodes two components of the small ribosomal subunit, 15S rRNA and the Var1 protein, both of which are necessary for the formation of mature 37S subunits. Several studies have shown that in the absence of Var1 incomplete subunits accumulate, which lack a limited number of ribosomal proteins. Here, we show that Mrp2 is one of the proteins absent from subunits lacking Var1, indicating that Var1 plays an important role in the incorporation of Mrp2 into mitochondrial ribosomal subunits.