Cell damage induced by photocatalysis of TiO2 thin films
Cell damage induced by photocatalysis of TiO2 thin films
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DOI：
10.1021/la034807r
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发表时间：
2003-10-14
期刊：
影响因子：
3.9
通讯作者：
Shen, P
中科院分区：
文献类型：
--
作者：
Lu, ZX;Zhou, L;Shen, P
在近紫外光照射下，二氧化钛（TiO2）表现出优异的杀菌活性。然而，存在一些不同的机制，细胞杀伤通过细菌。在本研究中，光催化杀菌TiO2薄膜的机制进行了研究，原子力显微镜（AFM）结合一些其他技术。首次用原子力显微镜直接观察到了细胞壁和细胞膜的分解过程。由此产生的细胞渗透性的变化通过钾离子（K+）渗漏来证实。量子点最初被设计为检测细胞对大分子的渗透性的探针。相应的杀菌活性的TiO2薄膜进行了检查，通过细胞活力测定。这些结果表明，细胞死亡是由于细胞壁和细胞膜的分解以及由此引起的细胞内分子的泄漏。
When illuminated by near-UV light, titanium dioxide (TiO2) exhibits excellent bactericidal activity. However, there exist some different mechanisms for cell killing via photocatalysis. In the present study, the photocatalytically bactericidal mechanism of TiO2 thin films was investigated by atomic force microscopy (AFM) in conjugation with some other techniques. The decomposition process of the cell wall and the cell membrane was directly observed by AFM for the first time. The resultant change in cell permeability was confirmed by potassium ion (K+) leakage. Quantum dots (QDs) were designed originally as a probe to examine the cell permeability for macromolecules. The corresponding bactericidal activity of TiO2 thin films was examined by cell viability assay. These results suggested that the cell death was caused by the decomposition of the cell wall and the cell membrane and the resultant leakage of intracellular molecules.