Regulated preparation of Crocin-1 or Crocin-2′ Triggered by the Cosolvent DMSO Using Bs-GT/At-SuSy One-Pot Reaction
Regulated preparation of Crocin-1 or Crocin-2′ Triggered by the Cosolvent DMSO Using Bs-GT/At-SuSy One-Pot Reaction
复制标题
使用 Bs-GT/At-SuSy 一锅反应通过共溶剂 DMSO 触发 Crocin-1 或 Crocin-2' 的调节制备
DOI：
10.1021/acs.jafc.9b05000
复制
发表时间：
2019-11-13
影响因子：
6.1
通讯作者：
Wang, Guangji
中科院分区：
文献类型：
--
作者：
Liu, Feng;Ding, Fangyu;Wang, Guangji
Crocins are the primary coloring ingredients of saffron. The low-glycosylated members of this compound family, such as crocin-1 (crocetin mono-glucosyl ester) and crocin-2' (crocetin di-glucosyl ester), are rarely distributed in nature and attracting interest for their therapeutic uses. In the present study, a one-pot reaction system was used for efficient preparation of crocin-1 and crocin-2' with in situ regeneration of UDP-Glc by coupling Bs-GT with At-SuSy, a sucrose synthase from Arabidopsis thaliana. Noticeably, DMSO was used as a cosolvent and resulted in improvement of the solubility of the substrate crocetin and regulation of the selectivity of glycosylation. With periodic addition of crocetin, the biosynthesis of crocin-2' was performed with a high yield of 3.25 g/L in 2% DMSO aqueous solution, whereas crocin-1 (2.12 g/L) was selectively obtained in a 10% DMSO aqueous solution. The present study provided a simple approach for the biosynthesis of crocin-1 and crocin-2'.
