Definitive hematopoietic commitment within the embryonic vascular endothelial-cadherin+ population
Definitive hematopoietic commitment within the embryonic vascular endothelial-cadherin+ population
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DOI：
10.1016/s0301-472x(02)00887-1
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发表时间：
2002-09-01
影响因子：
2.6
通讯作者：
Nishikawa, SI
中科院分区：
文献类型：
--
作者：
Fraser, ST;Ogawa, M;Nishikawa, SI
Objective.本研究的目的是评估胚胎发育不同阶段的FLK 1（+）和血管内皮（VE）-钙粘蛋白（+）群体体外产生造血细胞和促进受体小鼠造血系统的潜力。从7.5- 9.5dpc孕体分离VE-钙粘蛋白（+）细胞的FLK 1（+），并在OP 9基质细胞上离体培养，并检查造血发育。将8.5-dpc和9.5-dpc孕鼠的VE-cadherin（+）CD 45（-）细胞肝内注射到新生儿白消安治疗的SCID受体中并监测植入情况。与FLK 1（+）VE-钙粘蛋白（-）细胞相比，来自7.5-和8.5-dpc孕体的VE-钙粘蛋白（+）细胞可以容易地离体产生造血细胞。在来自8.5-dpc卵黄囊的VE-钙粘蛋白-细胞中可以发现类似的造血潜能。当VE-钙粘蛋白CD 45细胞被注射到SCID受体中时，观察到长期植入，特别是在淋巴系统内。在9.5-dpe胚胎或卵黄囊的VE-钙粘蛋白（+）CD 45细胞中观察到这种潜力，但在更年轻的妊娠组织中观察到这种潜力。FLK 1（+）VE-钙粘蛋白（-）细胞，可能代表侧板中胚层，与类似阶段的钙粘蛋白（+）细胞相比，在产生造血细胞方面不那么有效。VE-钙粘蛋白（+）CD 45（-）细胞也可以促进肝内注射的新生儿SCID受体的血淋巴系统，表明具有内皮表型的细胞能够产生长期的造血前体。(C)2002年国际实验血液学学会。出版社：Elsevier Science Inc.
Objective. The aim of this study was to assess the potential of FLK1(+) and vascular endothelial (VE)-cadherin(+) populations from different stages of embryonic development to generate hematopoietic cells ex vivo and to contribute to the hematopoietic systems of recipient mice.Materials and Methods. FLK1(+) of VE-cadherin(+) cells were isolated from 7.5- to 9.5.dpc concepti and cultured ex vivo on OP9 stromal cells and hematopoietic development examined. VE-cadherin (+)CD45(-) cells from 8.5- and 9.5-dpc concepti were injected intrahepatically into newborn busulfan-treated SCID recipients and engraftment monitored.Results. VE-cadherin(+) cells from 7.5- and 8.5-dpc concepti can readily generate hematopoietic cells ex vivo compared to FLK1(+) VE-cadherin(-) cells. Similar hematopoietic potential can be found in the VE-cadherin- cells from the 8.5-dpc yolk sac. When VE-cadherin CD45 cells were injected into SCID recipients, long-term engraftment, particularly, within the lymphoid system, was observed. This potential was observed in VE-cadherin(+) CD45 cells from 9.5-dpe embryo or yolk sac but from tissues from younger concepti.Conclusions. FLK1(+)VE-cadherin(-) cells, possibly representing the lateral plate mesoderm, are not as effective at generating hematopoietic cells compared to similarly staged cadherin(+) cells. VE-cadherin(+)CD45(-) cells can also contribute to the hematolymphoid system of intrahepatically injected newborn SCID recipients, suggesting that cells bearing an endothelial phenotype are capable of generating long-term hematopoietic precursors. (C) 2002 International Society for Experimental Hematology. Published by Elsevier Science Inc.