The pentatricopeptide repeat gene OTP43 is required for trans-splicing of the mitochondrial nad1 intron 1 in Arabidopsis thaliana
The pentatricopeptide repeat gene OTP43 is required for trans-splicing of the mitochondrial nad1 intron 1 in Arabidopsis thaliana
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DOI：
10.1105/tpc.107.054841
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发表时间：
2007-10-01
期刊：
影响因子：
11.6
通讯作者：
Small, Ian D.
中科院分区：
文献类型：
--
作者：
de Longevialle, Andeol Falcon;Meyer, Etienne H.;Small, Ian D.
线粒体NADH：泛醌氧化还原酶复合体（复合体I）是由核和线粒体编码亚基形成的大型蛋白质复合体。ND1亚基由一个包含五个外显子的线粒体基因编码，成熟的转录物需要四个RNA剪接事件，其中两个涉及反式剪接独立转录的RNA。我们已经确定了一个核基因（OTP43）是拟南芥nad1转录本内含子1（且仅内含子1）反式剪接所必需的。该基因编码一种以前未被表征的五肽重复蛋白。OTP43基因被破坏的拟南芥突变体没有可检测到的线粒体复合体I活性，在种子发育、萌发和较小程度的植物生长中表现出严重缺陷。涉及替代性氧化酶的替代性呼吸途径在突变体中被显著诱导。
The mitochondrial NADH: ubiquinone oxidoreductase complex (Complex I) is a large protein complex formed from both nuclearly and mitochondrially encoded subunits. Subunit ND1 is encoded by a mitochondrial gene comprising five exons, and the mature transcript requires four RNA splicing events, two of which involve trans-splicing independently transcribed RNAs. We have identified a nuclear gene (OTP43) absolutely required for trans-splicing of intron 1 (and only intron 1) of Arabidopsis thaliana nad1 transcripts. This gene encodes a previously uncharacterized pentatricopeptide repeat protein. Mutant Arabidopsis plants with a disrupted OTP43 gene do not present detectable mitochondrial Complex I activity and show severe defects in seed development, germination, and to a lesser extent in plant growth. The alternative respiratory pathway involving alternative oxidase is significantly induced in the mutant.