An improved ELISA for the detection of antibodies against Babesia bovis using either a native or a recombinant B. bovis antigen.
An improved ELISA for the detection of antibodies against Babesia bovis using either a native or a recombinant B. bovis antigen.
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DOI：
10.1007/bf00931081
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发表时间：
1990
影响因子：
2
通讯作者：
Wright IG
中科院分区：
文献类型：
--
作者：
Böse R;Jacobson RH;Gale KR;Waltisbuhl DJ;Wright IG
本文报道了两种新的诊断牛巴贝斯虫病的酶联免疫吸附试验(EL ISA)。使用天然抗原的酶联免疫吸附试验比之前描述的检测更灵敏、更省力，因为它不需要血清与牛红细胞的吸附。第二次酶联免疫吸附试验，使用重组子B。在大肠杆菌中表达的牛黄抗原具有较高的敏感性和特异性。它适合于替代天然抗原，从而避免了抗原制剂的大批量差异和牺牲实验牛的需要。
Two new enzyme-linked immunosorbent assayes (ELISA) for the diagnosis ofBabesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The second ELISA, using a recombinantB. bovis antigen expressed inEscherichia coli, was both sensitive and specific. It is suitable to replace the native antigen, thus avoiding large batch-to-batch variations in antigen preparations and the need to sacrifice experimental cattle.