CREATINE AND CONTROL OF MYOSIN SYNTHESIS IN DIFFERENTIATING SKELETAL-MUSCLE
CREATINE AND CONTROL OF MYOSIN SYNTHESIS IN DIFFERENTIATING SKELETAL-MUSCLE
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DOI：
10.1073/pnas.69.8.2250
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发表时间：
1972-01-01
影响因子：
11.1
通讯作者：
MORALES, MF
中科院分区：
文献类型：
--
作者：
INGWALL, JS;STOCKDAL.FE;MORALES, MF
这些实验证明肌酸是肌肉特有的收缩的最终产物，参与控制肌肉蛋白质的合成。体外给肌酸时，骨骼肌细胞在体外合成肌球蛋白重链内形成较快。在培养基中加入肌酸后的4小时内，这种反应是明显的，并且与10-100 μM肌酸的浓度有关。这种效应似乎对细胞特异性蛋白具有选择性，因为总蛋白合成速率不受影响。
These experiments provide evidence that creatine, an end product of contraction unique to muscle, is involved in the control of muscle-protein synthesis. Skeletal muscle cells formed bothin vitroandin vivosynthesize myosin heavy chain faster when supplied creatinein vitro. The response is apparent within four hours after addition of creatine to the culture medium, and is dependent on concentration over a range of 10-100 μM creatine. The effect seems to be selective for cell-specific proteins(s), since the rate of total protein synthesis is unaffected.