Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs
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DOI：
10.32607/20758251-2011-3-3-40-46
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发表时间：
2011-07-01
期刊：
影响因子：
2
通讯作者：
Atabekov, J. G.
中科院分区：
文献类型：
--
作者：
Arkhipenko, M. V.;Petrova, E. K.;Atabekov, J. G.
马铃薯X病毒（PVX）和其他一些马铃薯病毒可以用病毒外壳蛋白（CP）和RNA在体外重组。PVX CP不仅能与同源rna形成病毒核糖核蛋白复合物（vRNP），也能与外源rna形成病毒核糖核蛋白复合物（vRNP）。本文介绍了PVX CP和不同分类群的各种动植物病毒rna体外孵育后组装的vRNP的结构和性质。我们已经证明含有外源（异源）RNA的vRNP的形态和翻译性质与同源vRNP （PVX RNA PVX CP）相同。我们的数据表明，体外“混合”vRNP的组装可以从RNA的5'-近端区域开始，产生带有外源核酸的vRNP螺旋结构。PVX CP在体外形成的异源vRNP似乎不需要特定的5'端RNA核苷酸序列，PVX CP似乎能够将各种大小和组成的外源遗传物质包装成人工病毒样颗粒。
Potato virus X (PVX) and some other potexviruses can be reconstituted in vitro from viral coat protein (CP) and RNA. PVX CP is capable of forming viral ribonucleoprotein complexes (vRNP) not only with homologous, but also with foreign RNAs. This paper presents the structure and properties of vRNP assembled in vitro upon incubation of PVX CP and RNAs of various plant and animal viruses belonging to different taxonomic groups. We have shown that the morphology and translational properties of vRNPs containing foreign (heterologous) RNA are identical to those of homological vRNP (PVX RNA PVX CP). Our data suggest that the assembly of the "mixed" vRNP in vitro could be started at the 5'-proximal region of the RNA, producing a helical structure of vRNPs with foreign nucleic acids. The formation of heterologous vRNP in vitro with PVX CP appears not to require a specific 5' end RNA nucleotide sequence, and the PVX CP seems to be able to pack foreign genetic material of various sizes and compositions into artificial virus-like particles.