Structural and sequence diversity of the pathogenicity island of uropathogenic Escherichia coli which encodes the USP protein
Structural and sequence diversity of the pathogenicity island of uropathogenic Escherichia coli which encodes the USP protein
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DOI：
10.1016/s0378-1097(01)00442-6
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发表时间：
2001-11-27
影响因子：
2.1
通讯作者：
Kurazono, H
中科院分区：
文献类型：
--
作者：
Nakano, M;Yamamoto, S;Kurazono, H
对321株尿路致病性大肠埃希菌（UPEC）和12株大肠埃希菌（E.大肠杆菌分离自健康人的粪便样品，这些样品先前在使用USP（编码尿路致病特异性蛋白）DNA探针的菌落杂交试验中显示为阳性。通过PCR扩增检测usp基因的大小和毒力岛（PI）。观察到三种类型的大小变化的usp基因和四种类型的PI。对来自七个代表性菌株（六个UPEC和一个来自正常健康个体）的Pls的测序分析揭示，usp基因可分为两组，每组在3 '末端区域具有不同的序列。由usp下游的三个开放阅读框（ORF）编码的肽在C-末端区域具有相同的23个氨基酸残基。编码这些小ORF的亚区具有由六个片段组成的镶嵌结构。这些片段的位置因菌株而异。在某些菌株中，两到四个片段被删除。这表明重排经常发生在这个区域和镶嵌安排显然有助于在PCR检测usp基因和Pls中观察到的大小变化。(C)2001年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
A total of 321 uropathogenic Escherichia coli (UPEC) strains and 12 strains of E. coli isolated from stool samples of healthy individuals, which were previously shown to be positive in colony hybridization test using the usp (encoding for the uropathogenic-specific protein) DNA probe. were examined by PCR amplification to determine the size of the usp gene and the pathogenicity island (PI). Three types of size variation were observed for the usp gene and four types for the PI. Sequencing analysis of the Pls from seven representative strains (six UPEC and one from a normal healthy individual) revealed that the usp genes can be classified into two groups, each having different sequences in the 3'-terminal region. The peptides encoded by the three open reading frames (ORFs) downstream of usp had identical 23 amino acid residues in the C-terminal region. The subregion encoding these small ORFs has a mosaic structure constituted of six segments. The positions of these segments vary from strain to strain. and in some strains, two to four segments are deleted. This indicates that rearrangements occur frequently in this region and the mosaic arrangement apparently contributes to the size variation observed in the PCR examination of the usp genes and Pls. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.