Coating of Single DNA Molecules by Genetically Engineered Protein Diblock Copolymers
Coating of Single DNA Molecules by Genetically Engineered Protein Diblock Copolymers
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DOI：
10.1002/smll.201200939
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发表时间：
2012-11-19
期刊：
影响因子：
13.3
通讯作者：
de Vries, Renko
中科院分区：
文献类型：
--
作者：
Hernandez-Garcia, Armando;Werten, Marc W. T.;de Vries, Renko
包被 DNA 是调节其物理性质和相互作用的有效方法。目前的化学合成聚合物形成具有随机大小和形状的DNA聚集体。在这项研究中，在重组酵母中以高产率生产单分散蛋白质二嵌段共聚物。它们携带一个大的亲水性胶体块（大约 400 个氨基酸），连接到一个短的结合块（大约 12 个碱性氨基酸）。事实证明，这些蛋白质聚合物将单个 DNA 分子复合为高度稳定的纳米棒，让人想起圆柱形病毒。有人提出，连接到大胶体稳定块上的小尺寸结合块完全阻止了 DNA 分子的分子间和分子内桥接。这些蛋白质二嵌段作为支架，可以调整以应用于基于 DNA 的纳米技术。
Coating DNA is an effective way to modulate its physical properties and interactions. Current chemosynthetic polymers form DNA aggregates with random size and shape. In this study, monodisperse protein diblock copolymers are produced at high yield in recombinant yeast. They carry a large hydrophilic colloidal block (approximate to 400 amino acids) linked to a short binding block (approximate to 12 basic amino acids). It is demonstrated that these protein polymers complex single DNA molecules as highly stable nanorods, reminiscent of cylindrical viruses. It is proposed that inter- and intramolecular bridging of DNA molecules are prevented completely by the small size of the binding block attached to the large colloidal stability block. These protein diblocks serve as a scaffold that can be tuned for application in DNA-based nanotechnology.