Synthesis, degradation, and in vitro cell responses of sodium phosphate glasses for craniofacial bone repair
Synthesis, degradation, and in vitro cell responses of sodium phosphate glasses for craniofacial bone repair
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DOI：
10.1002/jbm.10020
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发表时间：
2002-03-05
期刊：
影响因子：
--
通讯作者：
Jones, IA
中科院分区：
文献类型：
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作者：
Gough, JE;Christian, P;Jones, IA
这份报告概述了用于拉伸纤维和制造可生物降解复合材料的磷酸钠玻璃的初步合成、降解和短期生物相容性。使用巨噬细胞系和原代人颅面部成骨细胞进行生物相容性研究。合成时间小于1h的磷酸氢钠和磷酸二氢钠玻璃的降解率高于合成时间大于等于3h的玻璃(0.015 mg cm~(-2)h~(-1))。磷酸氢和磷酸二氢的高比例和低比例玻璃的降解率非常相似。形成玻璃的冷凝路线应产生不同的降解率和不同的原料比例。结果表明，玻璃的降解速率与初始试剂的浓度无关，发生开环聚合，达到平衡状态。生物相容性研究表明，巨噬细胞活性最低(低水平的过氧化氢和白细胞介素1β释放和圆形形态)和高成骨细胞的生物相容性。我们研究的最终目的是生产一种生物相容的可溶性磷酸盐玻璃，这种玻璃可以被拉入纤维中，并融入到用于颅面骨修复的聚己内酯基质中。这份报告展示了一种与成骨细胞和巨噬细胞具有生物相容性的可溶玻璃的成功生产。我们实验室的最新数据表明，成功地拉伸并生产了一种新型的聚己内酯。(C)2001年John Wiley&Sons，Inc.生物医学杂志59：481-489,2002。
This report outlines the initial synthesis, degradation, and short-term biocompatibility of sodium phosphate glasses, for use in the drawing of fibers and manufacture of biodegradable composites. Biocompatibility studies were performed using a macrophage cell line and primary human craniofacial osteoblasts. Sodium hydrogen phosphate and sodium dihydrogen phosphate glass synthesized for less than 1 h, resulted in a higher degradation rate than glass synthesized for 3 h or more (0.015 Mg cm(-2) h(-1)). Glasses with high and low ratios of hydrogen phosphate to dihydrogen phosphate had very similar degradation rates. A condensation route for the formation of the glass should give rise to varying degradation rates with varying ratios of starting materials. It is suggested that the degradation rate of the glass is independent of the concentrations of the initial reagents and that ring-opening polymerization, which reaches an equilibrium state, occurs. Biocompatibility studies suggest minimal macrophage activation (low levels of peroxide and interleukin-1 beta release and rounded morphology) and high osteoblast biocompatibility. The ultimate aim of our studies is to produce a biocompatible soluble phosphate glass that can be drawn into fibers for incorporation into a polycaprolactone matrix for craniofacial bone repair. This report demonstrates the successful production of a soluble glass, which is biocompatible with regard to osteoblasts and macrophages. Recent data from our laboratory have demonstrated successful fiber drawing and production of a novel polycaprolactone. (C) 2001 John Wiley & Sons, Inc. J Biomed Mater Res 59: 481-489, 2002.