Changes in [3H]inositol-labelled phosphoinositides of pig platelets in response to thrombin.
Changes in [3H]inositol-labelled phosphoinositides of pig platelets in response to thrombin.
复制标题
猪血小板[3H]肌醇标记的磷酸肌醇响应凝血酶的变化。
DOI：
10.1016/0167-4889(84)90084-3
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
J. Hawthorne
中科院分区：
文献类型：
--
作者：
G. Lymberopoulos;J. Hawthorne
用[~3H]肌醇标记猪血小板磷脂酰肌醇，然后在无钙条件下用凝血酶处理。在加入凝血酶后，标记的磷脂酰肌醇4，5-二磷酸在30到60 S之间丢失，但总体情况是在4分钟内标记的增加。磷脂酰肌醇4-磷酸标记显示没有丢失期，但在4分钟孵育过程中，磷脂酰肌醇有早期丢失，标记没有增加。凝血酶治疗不影响血小板中少量的溶血磷脂酰肌醇。凝血酶导致[14C]花生四烯酸标记的磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰肌醇的丢失。
Pig platelet phosphoinositides have been labelled with [3H]inositol and then treated with thrombin in the absence of Ca2+. There was a loss of labelled phosphatidylinositol 4,5-bisphosphate between 30 and 60 s after the addition of thrombin but the general picture was of increased labelling over a 4-min period. Labelling of phosphatidylinositol 4-phosphate showed no period of loss but there was an early loss of phosphatidylinositol and no increased labelling during the 4-min incubation. The small amount of lysophosphatidyl[3H]inositol in the platelets was not affected by thrombin treatment. Thrombin caused loss of [14C]arachidonate-labelled phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol.