Differential location of different types of intermediate-sized filaments in various tissues of the chicken embryo.
Differential location of different types of intermediate-sized filaments in various tissues of the chicken embryo.
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鸡胚不同组织中不同类型的中等大小的丝的不同位置。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
Werner W. Franke
中科院分区：
文献类型：
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作者：
E. Schmid;Stephen Tapscott;GUDRUN S. Bennett;James Croop;STEVEN A. Fellin;Howard Holtzer;Werner W. Franke
用特异性抗体和免疫荧光显微镜，对11--20日龄鸡胚不同组织中的不同类型的中等大小（约10 mm）丝（cytokeratin，vimentin，desmin，brain filament protein）的组成蛋白进行了定位研究。所研究的含有上皮的组织是小肠、砂囊、食道、庄稼、肝、肾、胸腺、肠系膜和表皮。结果表明，在同一器官中所见的不同中间丝蛋白是特定分化系的特征：细胞角蛋白丝仅限于上皮细胞并对上皮细胞具有特异性;波形蛋白丝在胚胎发生的这个阶段仅见于间充质细胞，包括结缔组织、内皮细胞、血细胞和软骨细胞;含有与由砂囊10 nm细丝制备的亚基蛋白相关的蛋白质的细丝（即，结蛋白）仅在肌肉细胞中有意义;脑的中间丝蛋白，最可能是神经丝蛋白，仅存在于神经细胞中。我们的结论是，对于大多数组织的表达的细丝的细胞角蛋白，波形蛋白，结蛋白，和神经丝蛋白是相互排斥的，这些蛋白质structrees提供有用的标志物的组织化学和细胞化学分化的细胞的上皮，间充质，肌源性和神经源性分化。
The location of constitutive proteins of different types of intermediate-sized (about 10 mm) filaments (cytokeratin, vimentin, desmin, brain filament protein) was examined in various tissues of 11--20 day chick embryos, using specific antibodies against the isolated proteins and immunofluorescence microscopy on frozen sections and on isolated serous membrane. The tissues studied which contained epithelia were small intestine, gizzard, esophagus, crop, liver, kidney, thymus, mesenteries, and epidermis. The results show that the different intermediate filament proteins, as seen in the same organ, are characteristic of specific lines of differentiation: Cytokeratin filaments are restricted to--and specific for--epithelial cells; vimentin filaments are seen--at this stage of embryogenesis--only in mesenchymal cells, including connective tissue, endothelial and blood cells, and chondrocytes; filaments containing protein(s) related to the subunit protein prepared from gizzard 10 nm filaments (i.e., desmin) are significant only in muscle cells; and intermediate filament protein of brain, most probably neurofilament protein, is present only in nerve cells. We conclude that for most tissues the expression of filaments of cytokeratin, vimentin, desmin, and neurofilament protein is mutually exclusive, and that these protein structurees provide useful markers for histochemical and cytochemical differentiation of cells of epithelial, mesenchymal, myogenic, and neurogenic differentiation.