Degradation of acetochlor by a bacterial consortium of Rhodococcus sp.T3-1, Delftia sp.T3-6 and Sphingobium sp.MEA3-1
Degradation of acetochlor by a bacterial consortium of Rhodococcus sp.T3-1, Delftia sp.T3-6 and Sphingobium sp.MEA3-1
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DOI：
10.1111/lam.12242
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发表时间：
2014-07-01
影响因子：
2.4
通讯作者：
Cui, Z.
中科院分区：
文献类型：
--
作者：
Hou, Y.;Dong, W.;Cui, Z.
由于乙草胺在环境中的持久性及其对生态系统和人类健康的明显威胁，迫切需要寻找有效的方法去除乙草胺。本研究通过对乙草胺污染土壤的连续富集，获得了一株降解乙草胺的富集菌，命名为T3。T3在6d内可完全降解100 mgl-1乙草胺和丁草胺。分别以乙草胺和MEA为唯一碳源，从T3中分离鉴定出2株细菌Rhodococcus sp.T3-1和Delftia sp.T3-6，以及1株鞘氨醇菌Sphingobium sp.MEA3-1。这三种细菌通过协同代谢，可以将乙草胺完全矿化。根据降解中间产物的鉴定，提出了3株菌组成的复合体降解乙草胺的生化途径：Rhodococcus sp. T3-1降解乙草胺生成2-甲基-6-乙基-2-氯乙酰苯胺（CMEPA），Delftia sp. T3 -6降解CMEPA生成2-甲基-6-乙基苯胺（MEA），Sphingobium sp. MEA 3 -1降解MEA。在实验室条件下，该复合体对乙草胺的矿化是有效的。
Owing to acetochlor persistence in the environment and its perceptible threats to the ecosystem and human health, it is urgent to search for effective approaches to decontaminate acetochlor. In this study, an acetochlor-degrading enrichment culture was obtained by continuous enrichment from acetochlor-contaminated soil and named T3. T3 could completely degrade 100mgl-1 acetochlor and butachlor within 6days. Two bacterial strains Rhodococcus sp.T3-1 and Delftia sp.T3-6 and one strain Sphingobium sp.MEA3-1 were isolated and identified from T3 by using acetochlor and MEA as sole carbon source, respectively. These three bacteria could completely mineralize acetochlor by the cooperative metabolism. The biochemical pathway of acetochlor degradation by these three bacteria in a consortium was proposed: acetochlor to 2-methyl-6-ethyl-2-chloroacetanilide (CMEPA) by Rhodococcus sp. T3-1, CMEPA to 2-methyl-6-ethyl aniline (MEA) by Delftia sp.T3-6 and MEA by Sphingobium sp.MEA3-1 based on the identified degradation intermediates. Under laboratory conditions, the consortium was effective in the acetochlor mineralization.