Dietary oxidized fish oil negatively affected the feed utilization, health status and fillet quality of juvenile Amur sturgeon, A. schrenckii
Dietary oxidized fish oil negatively affected the feed utilization, health status and fillet quality of juvenile Amur sturgeon, A. schrenckii
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DOI：
10.1016/j.aquaculture.2021.737290
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发表时间：
2021-08-10
期刊：
影响因子：
4.5
通讯作者：
Luo, Lin
中科院分区：
文献类型：
--
作者：
Yu, Huanhuan;Ren, Yuanyuan;Luo, Lin
本试验旨在研究饲粮中添加氧化鱼油对施氏鲟幼鱼生长、饲料利用、健康状况和鱼片品质的影响。schrenckii）（初始平均体重= 70.0 +/- 0.02 g）。采用含有6%新鲜鱼油（FFO）或6%氧化鱼油（OFO）的饲料进行了为期8周的实验。新鲜鱼油和氧化鱼油的过氧化值分别为9.14和384.73 meq/kg。结果表明，与FFO组相比，日粮中添加OFO对生长无显著影响（P > 0.05），但显著提高了摄食率（P < 0.05），降低了生产蛋白值（P < 0.05）。与FFO组相比，OFO组的血清高密度脂蛋白胆固醇（HDL-C）、白蛋白（ALB）、白蛋白球蛋白比值（GLOB）降低，丙二醛（MDA）和超氧阴离子自由基（O-2（-））升高（P < 0.05）。OFO可诱导肝脏炎症细胞浸润，肝脏炎症细胞因子的表达显著上调（P < 0.05）。OFO组鱼体皮肤呈异常黄色，背肌黄度值显著高于FFO组（P < 0.05）。日粮中添加OFO显著降低了肌肉蛋白质、脂肪和糖原含量（P < 0.05）。日粮OFO显著影响肌肉必需氨基酸（EAA）组成，其中Ile和Met比例降低，Leu比例显著提高（P < 0.05）。OFO组鱼体n-6多不饱和脂肪酸（PUFA）比例和n-6 PUFA/n-3 PUFA比例显著高于FFO组（P < 0.05），而饱和脂肪酸（SFA）、单不饱和脂肪酸（MUFA）和n-3 PUFA比例无显著变化（P > 0.05）。OFO显著提高了肌肉中游离氨基酸总量（FAA），但降低了肌肉中鲜味氨基酸比例（DAA）（P < 0.05）。饲料中添加OFO对史氏鲟的生长没有显著影响，但降低了饲料利用率。OFO通过诱导氧化应激、降低血清HDL-C、ALB和引起肝脏损伤等途径损害了史氏鲟的健康状况。饲料中添加OFO对史氏鲟鱼糜品质的影响主要表现在体色、肌纤维组成、糖原含量、必需氨基酸、脂肪酸和游离氨基酸等方面。
This study was carried out to explore the effects of dietary oxidized fish oil on growth, feed utilization, health status and fillet quality of juvenile Amur sturgeon (A. schrenckii) (initial mean body weight = 70.0 +/- 0.02 g). An 8-week experiment with diets containing 6% fresh fish oil (FFO) or 6% oxidized fish oil (OFO) was conducted. The peroxide values (POV) of fresh and oxidized fish oil were 9.14 and 384.73 meq/kg, respectively. The results indicated that compared to the FFO group, dietary OFO had no significant influence on growth (P > 0.05), but increased feeding rate (FR) and decreased productive protein value (PPV) significantly (P < 0.05). The fish in the OFO group had lower serum high-density lipoprotein cholesterol (HDL-C), albumin (ALB), and the ratio of ALB to globulin (GLOB), and higher serum malonaldehyde (MDA) and superoxide anion free radical (O-2(-)) than those in the FFO group (P < 0.05). OFO induced inflammatory cell infiltration in the liver, and the expressions of hepatic inflammatory cytokines were significantly up regulated in the fish fed with OFO (P < 0.05). The fish in the OFO group showed abnormal yellow skin, and the yellowness value of the dorsal muscle was significantly elevated in comparison with the FFO group (P < 0.05). The contents of muscle protein, lipid, and glycogen were significantly declined by dietary OFO (P < 0.05). Dietary OFO affected the muscle essential amino acid (EAA) composition remarkably, among which the proportions of Ile and Met were reduced while Leu was promoted significantly (P < 0.05). The fish fed with OFO had significantly higher n-6 polyunsaturated fatty acids (PUFA) proportion and n-6 PUFA/n-3 PUFA ratio (P < 0.05) than the fish fed with FFO, but there were no changes in the proportion of saturated (SFA), monounsaturated fatty acid (MUFA) and n-3 PUFA (P > 0.05). The total free amino acid (FAA) was enhanced significantly by dietary OFO, but the delicious amino acid (DAA) proportion was decreased in the muscle of the fish fed with OFO (P < 0.05). In summary, dietary OFO had no significant influence on growth, but decreased feed utilization of Amur sturgeon. Dietary OFO impaired the health status of Amur sturgeon by inducing oxidative stress, lowering serum HDL-C, ALB and causing liver damage. Dietary OFO adversely affected the fillet quality of Amur sturgeon by altering body color, muscle proximate composition, glycogen content, EAA, fatty acid and FAA profiles.