A novel real-time PCR method for KIR genotyping
A novel real-time PCR method for KIR genotyping
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DOI：
10.1111/j.1399-0039.2008.01184.x
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发表时间：
2009-02-01
期刊：
影响因子：
--
通讯作者：
Canavez, F.
中科院分区：
文献类型：
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作者：
Alves, L. G. T.;Rajalingam, R.;Canavez, F.
编码杀伤细胞免疫球蛋白样受体（KIR）的基因在个体之间存在差异。序列特异性引物引导的聚合酶链式反应（PCR）扩增（PCR-SSP）和PCR扩增产物的序列特异性寡核苷酸杂交（PCR-SSO）是目前用于表征KIR基因内容多样性的方法。这两种方法都包括耗时的 PCR 后分析。在这里，我们开发了一种实时 PCR 方法，可在 PCR 过程中识别 16 个 KIR 基因是否存在，并避免 PCR 后分析。该方法特异、灵敏，与传统PCR-SSP和PCR-SSO方法相比缩短了周转时间，并且易于实现自动化。
Genes encoding killer cell immunoglobulin-like receptors (KIRs) are variable among individuals. Sequence-specific primer-directed polymerase chain reaction (PCR) amplification (PCR-SSP) and sequence-specific oligonucleotide hybridization of the PCR-amplified products (PCR-SSO) are the methods currently used to characterize the diversity of KIR gene content. Both these methods include time-consuming post-PCR analyses. Here, we developed a real-time PCR method that identifies the presence or absence of 16 KIR genes during PCR and avoids post-PCR analyses. This method is specific, sensitive, shortens the turnaround time compared with the conventional PCR-SSP and PCR-SSO methods, and it can be easily adapted for automation.