THE RELATIONSHIP BETWEEN ANTIGENIC STRUCTURE AND THE REQUIREMENT FOR THYMUS-DERIVED CELLS IN THE IMMUNE RESPONSE
THE RELATIONSHIP BETWEEN ANTIGENIC STRUCTURE AND THE REQUIREMENT FOR THYMUS-DERIVED CELLS IN THE IMMUNE RESPONSE
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抗原结构与免疫反应中胸腺来源细胞的需求之间的关系
DOI：
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发表时间：
1971
影响因子：
15.3
通讯作者：
A. Basten
中科院分区：
文献类型：
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作者：
M. Feldmann;A. Basten
某些抗原如聚合鞭毛蛋白能够在去胸腺小鼠中产生相对正常的抗体水平，而其他抗原，包括异源红细胞，则需要T细胞以辅助能力存在。通过比较ATXBM、XBM和正常小鼠脾脏细胞对阿德莱德沙门氏菌鞭毛抗原的原代IgM反应，探讨胸腺非依赖性抗体产生的机制。在ATXBM培养中，虽然相同的脾脏细胞对最佳剂量的羊红细胞没有反应，但在大剂量范围内，聚合鞭毛蛋白的抗体形成细胞水平没有降低。相比之下，当鞭毛决定因子以单体形式或作为鞭毛包裹的驴红细胞呈现时，观察到ATXBM小鼠脾脏细胞与XBM或正常对照之间的抗体反应存在高度显著差异。结果表明，在抗体生产中对T细胞的需求不是特异性抗原决定因素的特性，而是取决于抗原呈递的模式。这一结论的有效性通过使用另一个抗原决定因子（DNP）偶联胸腺非依赖性载体POL或胸腺依赖性载体DRC得到证实。来自XBM小鼠的脾细胞产生的AFC水平与两种形式的DNP相当，但来自ATXBM培养的结果显示出显着差异。与对照组相比，对DNP-DRC的抗dnp反应大大降低，而对DNP-POL的抗dnp反应正常，即使在对ATXBM供体进行长时间胸导管引流和用抗θ血清和补体预处理其脾细胞后也是如此。这里提供的数据表明，T细胞在体液免疫中的作用是将抗原呈递给B细胞，从而启动最佳的抗体合成。
Certain antigens such as polymerized flagellin are capable of producing relatively normal antibody levels in thymectomized mice, whereas others, including heterologous erythrocytes require the presence of T cells in a helper capacity. The mechanism of thymus-independent antibody production was investigated by comparing the primary IgM responses of spleen cells from ATXBM, XBM, and normal mice to various physical forms of the flagellar antigens of Salmonella adelaide in vitro. No reduction in antibody-forming cell levels to polymerized flagellin over a wide dose range was observed in ATXBM cultures, although the same spleen cells did not respond to an optimal dose of sheep red cells. In contrast, when flagellar determinants were presented in a monomeric form or as flagellin-coated donkey red cells, a highly significant difference was observed between the antibody responses of spleen cells from ATXBM mice and XBM or normal controls. The results suggested that the requirement for T cells in antibody production is not a property of specific antigenic determinants, but depends on the mode of antigenic presentation. The validity of this conclusion was confirmed by using another antigenic determinant (DNP) coupled either to the thymus-independent carrier, POL, or to the thymus-dependent carrier, DRC. Spleen cells from XBM mice produced comparable AFC levels to both forms of DNP, but the results from ATXBM cultures showed a marked difference. The anti-DNP response to DNP-DRC was greatly reduced compared to controls, whereas that to DNP-POL was normal even after prolonged thoracic duct drainage of the ATXBM donors and pretreatment of their spleen cells with anti-θ-serum and complement. The data presented here imply that the role of T cells in humoral immunity is the presentation of antigen to B cells in such a manner as to initiate optimal antibody synthesis.