Detection of novel swine origin influenza A virus (H1N1) by real-time nucleic acid sequence-based amplification
Detection of novel swine origin influenza A virus (H1N1) by real-time nucleic acid sequence-based amplification
复制标题
通过基于核酸序列的实时扩增检测新型猪源甲型流感病毒（H1N1）
DOI：
10.1016/j.jviromet.2009.10.025
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发表时间：
2010-02-01
影响因子：
3.1
通讯作者：
Shi, Zhiyang
中科院分区：
文献类型：
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作者：
Ge, Yiyue;Cui, Lunbiao;Shi, Zhiyang
快速检测新型猪源甲型流感病毒（S-OIV）（H1N1）对于及时实施感染控制措施至关重要。在这项研究中，血凝素（HA）基因为基础的实时核酸序列为基础的扩增（NASBA）检测特异性检测S-OIV（H1N1）。通过与现有的S-OIV（H1N1）检测方法进行比较，对该检测方法进行了评价和验证。在10倍稀释系列测定中获得的结果表明，本测定的分析灵敏度与商业S-OIV（H1N1）实时RT-PCR试剂盒相当，并且高于疾病控制和预防中心（CDC）TaqMan测定。实时NASBA测定的实际检测限为每个反应约50个拷贝。与参考方法（病毒培养、常规RT-PCR和实时RT-PCR）相比，本方法的敏感性、特异性、阳性预测值和阴性预测值均为100%。总体而言，结果表明，实时NASBA检测可用于敏感和特异性检测S-OIV（H1N1）。(C)2009爱思唯尔有限公司版权所有。
Rapid detection of novel swine origin influenza A virus (S-OIV) (H1N1) is crucial for timely implementation of infection control measures. In this study, a haemagglutinin (HA) gene-based real-time nucleic acid sequence-based amplification (NASBA) assay was developed for the specific detection of S-OIV (H1N1). The assay was evaluated and validated by comparing it with existing detection methods for S-OIV (H1N1). Results obtained in a 10-fold dilution series assay demonstrated the analytic sensitivity of the present assay was comparable to that of a commercial S-OIV (H1N1) real-time RT-PCR kit and higher than that of the Centers for Disease Control and Prevention (CDC) TaqMan assay. The actual detection limit of the real-time NASBA assay was approximately 50 copies per reaction. Compared with reference methods (viral culture, conventional RT-PCR, and real-time RT-PCR), the sensitivity, specificity, positive predictive value, and negative predictive value of the present assay were all 100%. Overall, the results showed that the real-time NASBA assay could be used for sensitive and specific detection of S-OIV (H1N1). (C) 2009 Elsevier B.V. All rights reserved.