Molecular cloning and functional analysis of Three FLOWERING LOCUS T (FT) homologous genes from Chinese Cymbidium.
Molecular cloning and functional analysis of Three FLOWERING LOCUS T (FT) homologous genes from Chinese Cymbidium.
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国兰三个FLOWERING LOCUS T(FT)同源基因的分子克隆及功能分析
DOI：
10.3390/ijms130911385
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发表时间：
2012
影响因子：
5.6
通讯作者：
Duan J
中科院分区：
文献类型：
--
作者：
Huang W;Fang Z;Zeng S;Zhang J;Wu K;Chen Z;Teixeira da Silva JA;Duan J
FLOW LOCUS T（FT）基因在调节从营养阶段到生殖阶段的转变中起着至关重要的作用。为了解建兰繁殖的分子机制，从建兰“七间白墨”、春兰和建兰“金丝马尾”中分离并鉴定了3个FT同源基因。这3个基因全长618 bp，开放阅读框（ORF）为531 bp，编码176个氨基酸。同源性分析表明，CsFT、CgFT和CeFT含有PEBP-RKIP超家族特有的保守结构域，与GenBank中其它植物FT的同源性较高：与文心兰OnFT的同源性为94%，与水稻Hd 3a的同源性为79%，与拟南芥FT的同源性为74%。qRT-PCR分析显示在长日照（LD，16小时光照/8小时黑暗）和短日照（SD，8小时光照/16小时黑暗）处理后CsFT、CgFT和CeFT的昼夜表达模式。LD处理下CsFT和CeFT的转录水平显著高于SD处理，而CgFT的转录水平高于SD处理。CgFT在转基因拟南芥植物中的异位表达导致了与野生型植物相比的早期开花和APETALA 1（AP 1）表达的显著上调。我们的数据表明，CgFT是一个假定的磷脂酰乙醇胺结合蛋白基因，在兰，可能调节营养繁殖的花过渡，类似于其拟南芥的直系同源物。
The FLOWERING LOCUS T (FT) gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. To understand the molecular mechanism of reproduction, three homologous FT genes were isolated and characterized from Cymbidium sinense “Qi Jian Bai Mo”, Cymbidium goeringii and Cymbidium ensifolium “Jin Si Ma Wei”. The three genes contained 618-bp nucleotides with a 531-bp open reading frame (ORF) of encoding 176 amino acids (AAs). Alignment of the AA sequences revealed that CsFT, CgFT and CeFT contain a conserved domain, which is characteristic of the PEBP-RKIP superfamily, and which share high identity with FT of other plants in GenBank: 94% with OnFT from Oncidium Gower Ramsey, 79% with Hd3a from Oryza sativa, and 74% with FT from Arabidopsis thaliana. qRT-PCR analysis showed a diurnal expression pattern of CsFT, CgFT and CeFT following both long day (LD, 16-h light/8-h dark) and short day (SD, 8-h light/16-h dark) treatment. While the transcripts of both CsFT and CeFT under LD were significantly higher than under SD, those of CgFT were higher under SD. Ectopic expression of CgFT in transgenic Arabidopsis plants resulted in early flowering compared to wild-type plants and significant up-regulation of APETALA1 (AP1) expression. Our data indicates that CgFT is a putative phosphatidylethanolamine-binding protein gene in Cymbidium that may regulate the vegetative to reproductive transition in flowers, similar to its Arabidopsis ortholog.
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影响因子：
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