CHARACTERIZATION OF A GENE CODING FOR A TYPE IIO BACTERIAL IGG-BINDING PROTEIN
CHARACTERIZATION OF A GENE CODING FOR A TYPE IIO BACTERIAL IGG-BINDING PROTEIN
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DOI：
10.1016/0161-5890(95)00022-7
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发表时间：
1995-06-01
影响因子：
3.6
通讯作者：
PODBIELSKI, A
中科院分区：
文献类型：
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作者：
BOYLE, MDP;WEBERHEYNEMANN, J;PODBIELSKI, A
A组链球菌可表达两类抗原性非免疫IgG结合蛋白。蛋白质的一个抗原组被针对克隆的fcr A基因产物制备的抗体（抗FcRA）识别。在本研究中，用于制备定义第二类抗原的抗体的免疫原显示为M血清型55 A组分离株A928的emm样（emmL）基因产物。emmL 55基因在大肠杆菌中表达。大肠杆菌产生类似于58，000分子的M（r），其以非免疫方式结合人IgG（1）、IgG（2）、IgG（3）和IgG（4）以及马、兔和猪IgE。这些性质是先前描述的从该菌株分离的IIo型IgG结合蛋白的特征。此外，重组蛋白与人血清白蛋白和纤维蛋白原反应。emmL 55基因序列进行了分析，发现具有典型的I类emm样基因的组织和序列特征。
Two antigenic classes of non-immune IgG-binding proteins can be expressed by group A streptococci. One antigenic group of proteins is recognized by an antibody prepared against the product of a cloned fcr A gene (anti-FcRA). In this study, the immunogen used to prepare the antibody that defines the second antigenic class was shown to be the product of the emm-like (emmL) gene of M serotype 55 group A isolate, A928. The emmL55 gene expressed in E. coli produced an M(r) similar to 58,000 molecule which bound human IgG(1), IgG(2), IgG(3), and IgG(4), as well as horse, rabbit and pig Ige in a non-immune fashion. These properties are characteristic of the previously described type IIo IgG-binding protein isolated from this strain. In addition, the recombinant protein was reactive with human serum albumin and fibrinogen. The emmL 55 gene sequence was analysed and found to have the organization and sequence characteristics of a typical class I emm-like gene.