Downregulation of miR-125b in metastatic cutaneous malignant melanoma
Downregulation of miR-125b in metastatic cutaneous malignant melanoma
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DOI：
10.1097/cmr.0b013e32833e32a1
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发表时间：
2010-12-01
影响因子：
2.2
通讯作者：
Drzewiecki, Krzysztof T.
中科院分区：
文献类型：
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作者：
Glud, Martin;Rossing, Maria;Drzewiecki, Krzysztof T.
本研究旨在鉴定参与皮肤恶性黑色素瘤（MM）最早转移事件的微小RNA种类。根据前哨淋巴结中是否存在微转移（N 0/N1）对28例MM患者[T2期（肿瘤），M0期（远处转移）]的样本进行分组。通过激光捕获显微切割从原发性皮肤MM肿瘤中收获黑素瘤细胞，并通过微阵列技术获得microRNA表达谱。通过定量逆转录PCR验证结果。我们发现，与前哨淋巴结阴性（T2，N 0，M0）黑色素瘤相比，miR-125 b在产生早期转移的原发性皮肤黑色素瘤（T2，N1，M0）中下调。MiR-125 b在其他肿瘤类型和寻常痣中的表达较常见的获得性痣下调。总之，miR-125 b可能参与皮肤MM的早期进展。Melanoma Res 20：479-484（C）2010 Wolters Kluwer Health vertical bar Lippincott威廉姆斯& Wilkins。
This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM. Melanoma Res 20:479-484 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.